宫颈永生化细胞和癌细胞的胞浆差异蛋白组学研究

宋大萍; 赵涌

doi:10.13406/j.cnki.cyxb.2010.12.029

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宫颈永生化细胞和癌细胞的胞浆差异蛋白组学研究

技术与方法 | 更新时间：2024-01-17

- 宫颈永生化细胞和癌细胞的胞浆差异蛋白组学研究
- Comparative proteomics analysis of cytoplasmic proteins of immortalized cervical cell and cervical cancer cell
- 重庆医科大学学报 2010年35卷第12期页码：1859-1862
- 作者机构：
  
  1. 绵阳市第三人民医院病理科
  2. 重庆医科大学基础学院病理学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.12.029
  中图分类号： R737.33
- 纸质出版日期：2010，
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[1]宋大萍,赵涌.宫颈永生化细胞和癌细胞的胞浆差异蛋白组学研究[J].重庆医科大学学报,2010,35(12):1859-1862.

SONG Da-ping, et al. Comparative proteomics analysis of cytoplasmic proteins of immortalized cervical cell and cervical cancer cell[J]. Journal of Chongqing Medical University, 2010,35(12):1859-1862.
[1]宋大萍,赵涌.宫颈永生化细胞和癌细胞的胞浆差异蛋白组学研究[J].重庆医科大学学报,2010,35(12):1859-1862. DOI： 10.13406/j.cnki.cyxb.2010.12.029.

SONG Da-ping, et al. Comparative proteomics analysis of cytoplasmic proteins of immortalized cervical cell and cervical cancer cell[J]. Journal of Chongqing Medical University, 2010,35(12):1859-1862. DOI： 10.13406/j.cnki.cyxb.2010.12.029.

摘要

目的:对HPV-16阳性的H8细胞株（宫颈上皮永生化细胞）和Caski细胞株（宫颈癌细胞）胞浆蛋白进行双向电泳和质谱分析

寻找与宫颈癌相关的特异性差异蛋白质。方法:采用双向凝胶电泳（2-dimensronal gel electrophoresis

2-DE）和基质辅助激光解吸飞行时间串联质谱（Matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry

MALDI-TOF-MS/MS）以及SWISS-PROT数据库

对H8和Caski细胞胞浆蛋白进行比较

找出差异蛋白质。结果:通过对宫颈永生化细胞和癌细胞胞浆蛋白2-DE图谱比较和质谱分析

初步鉴定出6个差异蛋白

它们分别为STK3、TRAF5、S100PI、KKb、CDK6、TAF2

其中S100P、IKKb、CDK6、TAF2、TRAF5这5种蛋白质在宫颈癌细胞胞浆中高表达

STK3蛋白质在宫颈癌细胞胞浆中表达缺失。结论:初步发现STK3、TRAF5、S100PI、KKb、CDK6、TAF2蛋白质在宫颈癌前病变和宫颈癌细胞胞浆中存在差异表达

通过对其功能的了解研究为宫颈癌前病变向癌转变机制的探讨、特异性肿瘤标志物的寻找提供实验依据。

Abstract

Objective:To identify differentially expressed protein spots of cytoplasm from H8 cells(human immortalized endocervical cell) and Caski(cervical cancer cell) associated with cervical carcinogenesis by two-dimensional electrophoresis(2-DE) analysis.Methods:The cytoplasmic proteins of H8 cell and Caski cell were separated by means of two-dimensional gel electrophoresis

then the selected differential protein spots were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) and SWISS-PROT database.Results:6 differentially expressed protein spots were successfully identified

among which

S100P

IKKb(Inhibitor of nuclear factor kappa-B kinase subunit beta)

CDK6(Cell division protein kinase 6)

TAF2(Transcription initiation factor TFIID subunit 2)

TRAF5(TNF receptor-associated factor 5) were significantly up-regulated in cytoplasmic proteins in Caski cell.STK3(Serine/threonine-protein kinase 3) deletion was found in cytoplasmic proteins in Caski cell.Conclusion:The differential expressions of proteins of cytoplasm may be valuable for studying mechanisms of cervical carcinogenesis

or as diagnostic markers and therapeutic targets for cervical cancer.

关键词

H8细胞CaSki细胞差异蛋白质2-DE基质辅助激光解吸飞行时间串联质谱

Keywords

H8 cellCaSki cellComparative proteomicsTwo-dimensional gel electrophoresisMALDI-TOF-MS

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