丹参酮ⅡA对人胆管癌HCCC-9810细胞增殖及VEGF表达的影响

周军; 郑军; 肖文波; 刘益军

doi:10.13406/j.cnki.cyxb.2010.12.024

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丹参酮ⅡA对人胆管癌HCCC-9810细胞增殖及VEGF表达的影响

基础研究 | 更新时间：2024-01-17

- 丹参酮ⅡA对人胆管癌HCCC-9810细胞增殖及VEGF表达的影响
- Influence of Tanshinone ⅡA on cell proliferation and vascular endothelial growth factor expression in human bile duct carcinoma cell line HCCC-9810
- 重庆医科大学学报 2010年35卷第12期页码：1798-1801
- 作者机构：
  
  重庆医科大学附属第一医院肝胆外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.12.024
  中图分类号： R735.8
- 纸质出版日期：2010，
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[1]周军,郑军,肖文波,刘益军.丹参酮ⅡA对人胆管癌HCCC-9810细胞增殖及VEGF表达的影响[J].重庆医科大学学报,2010,35(12):1798-1801.

ZHOU Jun, et al. Influence of Tanshinone ⅡA on cell proliferation and vascular endothelial growth factor expression in human bile duct carcinoma cell line HCCC-9810[J]. Journal of Chongqing Medical University, 2010,35(12):1798-1801.
[1]周军,郑军,肖文波,刘益军.丹参酮ⅡA对人胆管癌HCCC-9810细胞增殖及VEGF表达的影响[J].重庆医科大学学报,2010,35(12):1798-1801. DOI： 10.13406/j.cnki.cyxb.2010.12.024.

ZHOU Jun, et al. Influence of Tanshinone ⅡA on cell proliferation and vascular endothelial growth factor expression in human bile duct carcinoma cell line HCCC-9810[J]. Journal of Chongqing Medical University, 2010,35(12):1798-1801. DOI： 10.13406/j.cnki.cyxb.2010.12.024.

摘要

目的:观察丹参酮ⅡA（TanshinoneⅡA）对人胆管癌HCCC-9810细胞增殖和血管内皮生长因子（Vascular endothelial growth factor

VEGF）表达的影响

探讨其可能抗肿瘤的作用机制。方法:体外培养人胆管癌HCCC-9810细胞株

采用MTT比色法观察不同浓度和作用时间下丹参酮ⅡA对HCCC-9810细胞的增殖抑制情况;RT-PCR法检测细胞中VEGF mRNA的转录情况;Western blot法检测细胞内VEGF蛋白的表达。结果:低质量浓度丹参酮ⅡA（0.1 mg/L）对HCCC-9810细胞增殖抑制作用不明显

但当质量浓度达到0.25 mg/L以上时

则能显著抑制胆管癌细胞的增殖

其抑制效应随时间和浓度的增加呈逐渐增强

同时抑制癌细胞内VEGF mRNA的转录和VEGF蛋白的表达（P<0.01）。结论:丹参酮ⅡA在体外能显著抑制HCCC-9810细胞增殖

并能抑制VEGF基因表达

提示这可能是其抗肿瘤的作用机制之一。

Abstract

Objective:To observe the influence of Tanshinone ⅡA on cell proliferation and vascular endothelial growth factor expression in human bile duct carcinoma cell line HCCC-9810

and to explore its possible anti-tumor mechanism of action.Methods:Human bile duct carcinoma HCCC-9810 cells were cultured in vitro and treated with tanshinone ⅡA.The cell proliferation was examined by the method of MTT;the transcriptions of VEGF mRNA were demonstrated by RT-PCR technique;Western blot was applied to examine the protein expression of VEGF.Results:Low-dose(0.1 mg/L)tanshinone ⅡA has no significant inhibitory effect on cellular proliferation of HCCC-9810 cells.When the concentration exceeded 0.25 mg/L

tanshinone ⅡA significantly inhibited cellular proliferation in a time-and concentration-dependent manner

and down regulated the mRNA and protein expression of VEGF(P<0.01).Conclusion:Within a certain drug concentration

tanshinone ⅡA can inhibit the proliferation of HCCC-9810 cells and play a potential role in inhibiting angiogenesis by down-regulating the expression of VEGF.

关键词

丹参酮ⅡA胆管癌血管内皮生长因子

Keywords

Tanshinone ⅡABile duct carcinomaVEGF

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