WEI Wei, et al. Effects of myricetin on the apoptosis of bladder cancer cell BIU-87[J]. Journal of Chongqing Medical University, 2010,35(12):1791-1794.
WEI Wei, et al. Effects of myricetin on the apoptosis of bladder cancer cell BIU-87[J]. Journal of Chongqing Medical University, 2010,35(12):1791-1794. DOI: 10.13406/j.cnki.cyxb.2010.12.023.
Objective:To explore the apoptosis effect and mechanism of myricetin on human bladder cancer BIU-87 cell line.Methods:BIU-87 cell was cultured and induced with myricetin of concentration gradient.The effect of myricetin on BIU-87 cell apoptosis was observed by MTT and Hoechst staining on inverted microscope.The levels of survivin and caspase-3 mRNA were detected by RT-PCR.The proteins of survivin and caspase-3 were analyzed by Western blot.Results:It showed that myricetin remarkably induced apoptosis of BIU-87 cell line.Moreover
myricetin suppressed survivin and up-regulated caspase-3 expression.Conclusion:The apoptosis mechanism of myricetin on human bladder cancer BIU-87 cell line involves decreasing the survivin expression and enhancing the expression and activity of caspase-3.