Objective:To investigate the pathogenesis of chronic pancreatitis(CP) by establishing a new pancreatitis model in rats suitable for investigation of the processes and mechanisms leading to pancreatic fibrosis.The model was established with ethanol and lipopolysaccharides(LPS

endotoxin).Methods:Eighteen one-month old male SD rats were randomly divided into control group

alcohol group

and model group.After fed with 25% alcohol for twelve weeks

the rats in model group were injected with lipopolysaccharides(LPS).Pancreatic morphology was conducted with haematoxylin-eosin stain

collagen deposition was determined by Masson stain

and the amylase and lipase in pancreatic tissue homogenate were evaluated by colorimetry.Results:Compared with control group

the acinar cells with vacuolization

inflammatory cell infiltration and collagen increasing were observed in the model group.Lipase and amylase were significantly decreased in the model group(P<0.05).Conclusion:Combined with ethanol

LPS can induce chronic pancreatitis.The rat model may be useful for studying the mechanism of chronic pancreatitis.