Objective:To investigate the value of morphology

immunophenotype

and combined analysis of both for primary diagnosis of acute myeloid leukemia(AML).in order to conduct the diagnosis of AML by flow cytometry and raise the diagnostic accuracy.Methods:A retrospective study on cell morphology and immunophenotype of 113 cases of established AML was performed.Immunophenotype analysis was carried out by flow cytometry

and 23 kinds of immune fluorescence marked McAb were used to detect the antigen expression of leukemia cells.Results:The coincidence between the morphology type and the final diagnosis was79.4%

that between immunophenotype and the final diagnosis was 85.3%.When morphology type and immunophenotype were combined

the final coincidence rate was 96.5%.The expression rates of modularly system antigen CD117

CD13

and CD33 were high

but with no specificity.cMPO was expressed in almost each morphology type except M0.The expressions of CD14 and CD64 in type M4 and M5 were higher than those in other types.The expressions of CD34 and HLADR in type M3 were lower than those in other types.Lymphatic system antigen was expressed in every type of AML

mainly CD4

CD7

CD9

CD10

CD19

and CD56.Conclusion:The antigen expression in each type of AML was complicated and with high heterogeneity.Analysis with enough antigens and the scatter situation of cells in Gate SSC/CD45 would be helpful for judging the origin

type

and phase of leukemic cells. Because of many influencing factors

the counting by flow cytometry is not as exact as classification by morphology.Analysis of morphology combined with immunophenotype can improve the accuracy of diagnosis and classification.