pshRNA-MDRla联合TRAIL基因对胃癌细胞株SGC7901/VCR作用及其作用机制的实验研究

袁媛; 周洲; 周静; 姜政; 黄爱龙; 王丕龙

doi:10.13406/j.cnki.cyxb.2010.11.034

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pshRNA-MDRla联合TRAIL基因对胃癌细胞株SGC7901/VCR作用及其作用机制的实验研究

基础研究 | 更新时间：2024-01-17

- pshRNA-MDRla联合TRAIL基因对胃癌细胞株SGC7901/VCR作用及其作用机制的实验研究
- Experimental study on the effects of pshRNA-MDRla combined with pBUD-TRAIL in inducing SGC7901/VCR cell apoptosis and proliferation and their mechanism
- 重庆医科大学学报 2010年35卷第11期页码：1646-1649
- 作者机构：
  
  1. 重庆医科大学附属第一医院消化科
  2. 重庆医科大学病毒性肝炎研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.034
  中图分类号： R735.2
- 纸质出版日期：2010，
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[1]袁媛,周洲,周静,姜政,黄爱龙,王丕龙.pshRNA-MDRla联合TRAIL基因对胃癌细胞株SGC7901/VCR作用及其作用机制的实验研究[J].重庆医科大学学报,2010,35(11):1646-1649.

YUAN Yuan, et al. Experimental study on the effects of pshRNA-MDRla combined with pBUD-TRAIL in inducing SGC7901/VCR cell apoptosis and proliferation and their mechanism[J]. Journal of Chongqing Medical University, 2010,35(11):1646-1649.
[1]袁媛,周洲,周静,姜政,黄爱龙,王丕龙.pshRNA-MDRla联合TRAIL基因对胃癌细胞株SGC7901/VCR作用及其作用机制的实验研究[J].重庆医科大学学报,2010,35(11):1646-1649. DOI： 10.13406/j.cnki.cyxb.2010.11.034.

YUAN Yuan, et al. Experimental study on the effects of pshRNA-MDRla combined with pBUD-TRAIL in inducing SGC7901/VCR cell apoptosis and proliferation and their mechanism[J]. Journal of Chongqing Medical University, 2010,35(11):1646-1649. DOI： 10.13406/j.cnki.cyxb.2010.11.034.

摘要

目的:探讨pshRNA-MDRla联合pBUD-TRAIL

在体外对胃癌细胞SGC7901/VCR增殖和凋亡的作用及机制

为肿瘤的基因治疗奠定基础。方法:以人胃癌SGC7901/VCR细胞株为研究对象

将pshRNA-MDRla与pBUD-TRAIL共同转染胃癌SGC7901/VCR细胞并在VCR中培养

同时与pshRNA-MDRla组、pBUD-TRAIL组、SGC7901/VCR和空质粒组相比较

以MTT法检测细胞增殖抑制率、以FCM检测细胞凋亡率以及TUNEL法检测细胞凋亡等

观察联合应用组对肿瘤细胞增殖的抑制作用。结果:MTT法显示:对照组（pBUD-TRAIL、pshRNA-MDRla、空质粒和SGC7901/VCR组）胃癌细胞的生长抑制率分别为45%、30%、10%和10%

联合实验组抑制率为70%;FCM检测显示:对照组胃癌细胞的凋亡率分别为25.96%、10.27%、5.96%和4.25%

联合实验组凋亡率为41.23%;与对照组相比

联合实验组抑制率和凋亡率都具有显著性差异;TUNEL检测结果显示:联合组与其他对照组相比较

细胞核明显棕黄色深染

且阳性染色细胞数目明显增多

表明pBUD-TRAIL与pshRNA-MDRla联合作用

凋亡细胞明显增加。结论:pshRNA-MDRla联合pBUD-TRAIL可显著抑制肿瘤细胞的增殖和促进肿瘤细胞的凋亡

推测与pshRNA-MDRla阻止TRAIL外排有关

从而增强TRAIL促进肿瘤细胞凋亡的作用

两者具有协同作用。

Abstract

Objective:To investigate the effects of pshRNA-MDRla combined with TRAIL in treating gastric cancer cell lines SGC7901/VCR and understand their mechanism

in order to lay experimental and theoretical foundation for clinical application of gene tumor therapy.Methods:SGC7901/VCR cells transfected with pBUD-TRAIL and pshRNA-MDRla simultaneously were cultivated with 1mg/L concentration of VCR

and compared with SGC7901/VCR cell transfected with pshRNA-MDRla

pBUD-TRAIL

pBUD groups and SGC7901/VCR group

respectively.The inhibition of cell proliferation was examined by methylthiazolyl tetrazolium(MTT) method

and cell apoptosis was detected by TUNEL method and flow cytometry using PI staining analysis.Results:The inhibition rates in pshRNA-MDRla

pBUD-TRAIL

pBUD and SGC7901/VCR groups were respectively 45%

30%

10%

and 10% by MTT.The apoptosis rates in pshRNA-MDRla

pBUD-TRAIL

pBUD and SGC7901/VCR groups were respectively 25.96%

10.27%

5.96%

and 4.25% by FCM.Compared with control group

the inhibition rate of the proliferation and apoptosis rate of SGC7901/VCR cells in pshRNA-MDRla combinated with TRAIL group was higher(70%

41.23% respectively);Karyopycnosis

nuclear chromosomal condensation and segmentation were observed by TUNEL.pshRNA-MDRla combinated with TRAIL group could significantly induce the apoptosis of tumor.Conclusion:pshRNA-MDRla combined with TRAIL could inhibit the proliferation and induce apoptosis of SGC7901/VCR cells

which could be related with the interfering effects of pshRNA-MDRla by prohibiting TRAIL excretion

so as to intensify the effect of TRAIL in promoting tumor cell apoptosis and have a synergistic action with TRAIL.

关键词

pshRNA-MDRla肿瘤坏死因子相关的凋亡诱导配体增殖凋亡

Keywords

pshRNA-MDRlaTRAILproliferationapoptosis

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