SB203580对大鼠慢性非细菌性前列腺炎作用的研究

李时军; 崔应东; 何昀; 张唯力

doi:10.13406/j.cnki.cyxb.2010.11.028

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SB203580对大鼠慢性非细菌性前列腺炎作用的研究

基础研究 | 更新时间：2024-01-17

- SB203580对大鼠慢性非细菌性前列腺炎作用的研究
- Effect of SB203580 on rat chronic nonbacterial prostatitis
- 重庆医科大学学报 2010年35卷第11期页码：1654-1657
- 作者机构：
  
  1. 湖北省恩施自治州中心医院(中医部)泌尿外科
  2. 重庆医科大学附属第二医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.028
  中图分类号： R697.33
- 纸质出版日期：2010，
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[1]李时军,崔应东,何昀,张唯力.SB203580对大鼠慢性非细菌性前列腺炎作用的研究[J].重庆医科大学学报,2010,35(11):1654-1657.

LI Shi-jun, et al. Effect of SB203580 on rat chronic nonbacterial prostatitis[J]. Journal of Chongqing Medical University, 2010,35(11):1654-1657.
[1]李时军,崔应东,何昀,张唯力.SB203580对大鼠慢性非细菌性前列腺炎作用的研究[J].重庆医科大学学报,2010,35(11):1654-1657. DOI： 10.13406/j.cnki.cyxb.2010.11.028.

LI Shi-jun, et al. Effect of SB203580 on rat chronic nonbacterial prostatitis[J]. Journal of Chongqing Medical University, 2010,35(11):1654-1657. DOI： 10.13406/j.cnki.cyxb.2010.11.028.

摘要

目的:探讨p38丝裂原活化蛋白激酶（p38 mitogen activated protein kinase

p38MAPK）特异性抑制剂SB203580对大鼠慢性非细菌性前列腺炎（Chronic nonbacterial prostatitis

CNP）的作用

及对细胞因子肿瘤坏死因子α（Tumor necrosis factor α

TNF-α）、基质金属蛋白酶（Matrix metalloproteinase 9

MMP-9）、环氧合酶（Cyclooxygenase 2

COX-2）表达的影响。方法:将成年雄性SD大鼠随机分为3组

炎症组和处理组去势后每日皮下注射苯甲酸雌二醇连续4周

同时处理组腹腔注射SB203580隔日1次

对照组不做任何处理。HE染色观察前列腺组织的病理学改变

免疫组化观察TNF-α、MMP-9、COX-2、磷酸化p38（Phosphorylation p38

p-p38）表达

Western blot和RT-PCR检测各组TNF-α、MMP-9、COX-2、p38（p-p38）的表达。结果:光镜下SB203580处理组较炎症组的炎症轻。p38 mRNA的表达在SB203580处理组明显比炎症组低。炎症组TNF-α、MMP-9、COX-2、p-p38蛋白呈高表达

应用SB203580表达显著降低与模型组有差异

与对照组无显著差异。结论:p38 MAPK信号转导途径在CNP中被激活

SB203580能有效地抑制炎症细胞因子TNF-α、MMP-9、COX-2等表达的调控

缓解炎症

这为CNP的治疗提供了新的理论和实验依据。

Abstract

Objective:To investigate the effect of SB203580

a specific inhibitor of mitogen activated protein kinase p38

on rat chronic nonbacteral prostatitis and its effect on the expressions of TNF-α

MMP-9 and COX-2.Methods:Adult male SD rats were randomly divided into three groups

model and treated groups were castrated and hypodermically injected with benzestrofol for 4 weeks;treated group was also injected with SB203580 intraperitoneally every two days;then control group was set up without any treatment.H.E staining pathology of prostate gland tissue was observed under light microscope.The expressions of TNF-α

MMP-9

COX-2 and p-p38 in each group were checked by immunohistochemistry

Western blot and RT-PCR respectively.Results:Inflammatory reaction was severer in model group than SB203580 treated group.Expression of p38 mRNA in model group was higher than those in other two groups.The protein expressions of TNF-α

MMP-9

COX-2 and p-p38 were lower in control group than those in model group.After treated with SB203580

the expressions of all the four proteins decreased significantly and could be compared to those in control group.Conclusion:p38 MAPK is activated in CNP.SB203580 can effectively inhibit expressions of TNF-α

MMP-9 and COX-2

and relieve inflammation

which provides an experimental evidence for new treatment of CNP.

关键词

p38MAPKSB203580慢性前列腺炎细胞因子

Keywords

p38 MAPKSB203580Chronic nonbacterial prostatitiscytokine

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