大鼠Meynert核神经元钠钾钙电流观察

朱淑娟; 钱亦华; 史利利; 张萌涛; 胡晓丹

doi:10.13406/j.cnki.cyxb.2010.10.006

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大鼠Meynert核神经元钠钾钙电流观察

基础研究 | 更新时间：2024-01-17

- 大鼠Meynert核神经元钠钾钙电流观察
- The observation of sodium current,potassium current and calcium current on the neurons of nucleus basalis of Meynert in rat
- 重庆医科大学学报 2010年35卷第10期页码：1479-1482
- 作者机构：
  
  1. 重庆医科大学神经科学研究中心
  2. 西安交通大学医学院人体解剖与组织胚胎学系
- 作者简介：
- 基金信息：
  
  西安市科技局资助项目（编号:GG04145、GG06175）
- DOI：10.13406/j.cnki.cyxb.2010.10.006
  中图分类号： R338
- 纸质出版日期：2010，
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[1]朱淑娟,钱亦华,史利利,张萌涛,胡晓丹.大鼠Meynert核神经元钠钾钙电流观察[J].重庆医科大学学报,2010,35(10):1479-1482.

ZHU Shu-juan, et al. The observation of sodium current,potassium current and calcium current on the neurons of nucleus basalis of Meynert in rat[J]. Journal of Chongqing Medical University, 2010,35(10):1479-1482.
[1]朱淑娟,钱亦华,史利利,张萌涛,胡晓丹.大鼠Meynert核神经元钠钾钙电流观察[J].重庆医科大学学报,2010,35(10):1479-1482. DOI： 10.13406/j.cnki.cyxb.2010.10.006.

ZHU Shu-juan, et al. The observation of sodium current,potassium current and calcium current on the neurons of nucleus basalis of Meynert in rat[J]. Journal of Chongqing Medical University, 2010,35(10):1479-1482. DOI： 10.13406/j.cnki.cyxb.2010.10.006.

摘要

目的:检测Meynert核团（Nucless basalis of meynert

nbM）神经细胞膜上钠、钾、钙通道电流。方法:采用细胞急性分离技术获得单个神经元

运用单细胞膜片钳技术全细胞记录方式记录钠、钾、钙通道电流。结果:钠电流激活失活均很迅速-

50 mV时峰值电流达到最大

约3～4 nA;钾离子电流分为瞬时外向钾电流（IA）和延迟整流性钾电流（IK）两部分I

A部分失活较快I

k部分几乎不失活

峰值电流最大约为7～8 nA;记录出内向的钙电流

失活较慢

大约0～10 mV时峰值电流达到最大

通过运用CdCl2证明该电流为Ca2+离子所介导的高电位激活性钙电流。结论:在nbM神经元上能够诱导出钠、钾、钙电压门控通道电流

为进一步相关研究提供了实验依据。

Abstract

Objective:Torecord normal sodiumcurrent

potassiumcurrent

and calciumcurrent on neurons ofnucleus basalis of Meynert (nbM). Methods:Single-cell was obtained with cell acutely dissociated technique

sodium current

potassium current

and calcium current were recorded by patch-clamp technique of single cell under whole cell patch pattern. Results:The activation and inactivation of sodium current were showed swiftly. The sodium peak current reached maximum

about 3~4 nA at-50 mV. Potassium current included transient outward potassium currents (I A )and delayed rectifier-like potassium currents (I k ). I A was activated swiftly

I k was hardly inactivated. The Potassium peak current was about 7~8 nA. The inward calcium current was inactivated slowly. The calcium peak current reached maximum

whilethevoltagewas0~10 mV.Thecurrentwasblocked by20 μmol/LCdCl 2 completely. Conclusion:The voltage-gated sodium channel current

voltage-gated potassium channel current

and voltage-gated calcium channel current were recorded successfully. It provided experimental data for further research.

关键词

膜片钳Meynert核钠、钾、钙电流

Keywords

patch clampNucleus basalis ofMeynertSodiumcurrentpotassiumcurrentcalciumcurrent

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