Objective:To search for association of heart period signal spectrum features in the supine posture with single nucleotide polymorphisms of three sites of klotho G-395A

F352V and C370S in Chinese Han nationality population. Method s:Genomic DNA of white blood cells from 177 individuals as research objects ( female 81 and male 96

age 65.11±10.7 y)selected randomly from Chinese Han nationality population was gotten. The SNPs of klotho G-395A

F352V and C370S were genotyped by allele-specific primer(ASP) PCR technique. We applied the analytical software for spectral features of heart period signal (HPS)to determine the spectral parameters of heart period signal in the supine posture in this group. Results:①The spectral parameters of HPS including absolute high frequency power(AHP)and relative high frequency powe(rRHP)in the group with AA genotype were significantly low than those in the group with GA genetype of klotho G-395A ( P<0.05). The spectral parameter of relative high frequency power(RHP)in the group with AA genotype were significantly lower than those in the group with GG genetype of klotho G-395A(P<0.05). ②The spectral parameters of HPS including relative ultra-lower frequency power(RUP)and ratio of AUP to AHP(RUH)in the group with VV genotype were significantly lower than those in the group with FF genetype of klotho F352V(P<0.05). The spectral parameters of absolute low frequency powe(rALP)

absolute high frequency power(AHP)

relative high frequency power(RHP)and total power value(TPV)in the group carrying VV genetype were significantly higher than those in the group with FF genetype of klotho F352V(P<0.05). The spectral parameters of RUP and RUH in the group with VV genotype were obviously lower than those in the group with FV genetype of klotho F352V and the value of RHP in the group with VV genotype were also much higher than that in the group with FV genetype of klotho F352V(P<0.05). ③The value of ratio of ALP to AHP (RLH)in the group with SS genotype was significantly lower than that in the group with CC genetype and the group carrying with CS genetype of klotho C370S (P<0.05). Conclusion:The AA genetype ofKlotho G-395A was associated with the decrease ofAHP and RHP parameters in Chinese Han Nationality Population. The mutated VV genetype of klotho F352V was not only associated with the decrease of RUP and RUH parameters but also with the increase of ALP

AHP

RHP and TPV parameters in Chinese Han Nationality Population. This suggests that there be an identify relationship between single nucleotide polymorphism of klotho gene and heart period signal spectrum features in Chinese Han Nationality Population.