Objective:To explore the damage of hyperglycemia to the neurons.Methods:Adult male SD rats were randomly divided into 3 groups:normal control group

traumatic brain injury group and insulin treated group

the blood glucose concentrations of the rats were measured before injury and when they were killed.The method of RT-PCR was applied to detect the expression of GLUT-3 genes.The change of GLUT-3 protein was detected by western-blot.The changes of NSE and NSE positive-Staining cells were examined by immunofluorescence staining Longa

balance test and screen test score were used to assess neural fnuctional defect.Results:The blood glucose concentration only increased markedly in traumatic brain injury group.through the expression of GLUT-3 and single cell NSE increased in the injured cortical both in traumatic brain injury group and insulin treated group

there was much more expression of GLUT-3 and single cell NSE but less NSE positive-Staining cells and neural functional defect in the insulin treated group at 12

24

48

72 h after injury.Meanwhile there were no changes were found in the uninjured side of each group.Conclusion:The hyperglycemia after traumatic brain injury increased the damage to the neurons by decreasing the expression of GLUT-3.