外源性重组S100A6蛋白对5株转化细胞系的增殖、迁移能力及β-catenin水平的影响

马闻; 李星星; 王胜; 游莉; 郭元元; 徐兰兰; 周兰

doi:10.13406/j.cnki.cyxb.2010.09.007

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外源性重组S100A6蛋白对5株转化细胞系的增殖、迁移能力及β-catenin水平的影响

基础研究 | 更新时间：2024-01-17

- 外源性重组S100A6蛋白对5株转化细胞系的增殖、迁移能力及β-catenin水平的影响
- Effects of exogenetic recombinant protein S100A6 on proliferation and migration of 5 transformed cell lines and β-catenin expression level
- 重庆医科大学学报 2010年35卷第9期页码：1321-1326
- 作者机构：
  
  1. 重庆医科大学生物医学工程系
  2. 重庆医科大学实验动物中心
  3. 重庆医科大学医学检验系临床检验诊断学、教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.09.007
  中图分类号： R329
- 纸质出版日期：2010，
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[1]马闻,李星星,王胜,游莉,郭元元,徐兰兰,周兰.外源性重组S100A6蛋白对5株转化细胞系的增殖、迁移能力及β-catenin水平的影响[J].重庆医科大学学报,2010,35(09):1321-1326.

MA Wen, et al. Effects of exogenetic recombinant protein S100A6 on proliferation and migration of 5 transformed cell lines and β-catenin expression level[J]. Journal of Chongqing Medical University, 2010,35(9):1321-1326.
[1]马闻,李星星,王胜,游莉,郭元元,徐兰兰,周兰.外源性重组S100A6蛋白对5株转化细胞系的增殖、迁移能力及β-catenin水平的影响[J].重庆医科大学学报,2010,35(09):1321-1326. DOI： 10.13406/j.cnki.cyxb.2010.09.007.

MA Wen, et al. Effects of exogenetic recombinant protein S100A6 on proliferation and migration of 5 transformed cell lines and β-catenin expression level[J]. Journal of Chongqing Medical University, 2010,35(9):1321-1326. DOI： 10.13406/j.cnki.cyxb.2010.09.007.

摘要

目的:探讨S100A6对5种转化细胞系株（HEK293、9HTE、CHO、MEF和C3H10T1/2）的增殖、迁移能力和β-catenin水平的影响。方法:通过转化质粒pMoluc-GST-hS100A6到大肠杆菌BL21

经IPTG诱导表达重组S100A6蛋白

为后续实验提供材料;MTT法检测细胞增殖

细胞划痕和Transwell法检测细胞迁移

免疫细胞化学法及Western-blotting检测β-catenin表达水平。结果:①通过IPTG诱导表达

从大肠杆菌中收获了高纯度重组S100A6（GST-S100A6）;②外源性重组S100A6对作为阳性对照的肿瘤细胞系——人骨肉瘤细胞143B的增殖及迁移均有抑制作用（P<0.05）

而对5株转化细胞系的增殖及迁移均无显著影响（P>0.05）;③免疫细胞化学法和Western-blotting一致揭示重组S100A6可上调5株转化细胞系的β-catenin水平（P<0.05）。结论:S100A6对5株转化细胞系的增殖、迁移无明显影响

但可上调此5株转化细胞系Wnt/β-catenin水平。

Abstract

Objective:This study aimed to investigate effects of exogenetic recombinant protein S100A6 on 5 transformed cells(HEK293

9HTE

CHO

MEF and C3H10T1/2) and their β-catenin expression level.Methods:Plasmid pMoluc-GST-hS100A6 was transformed into E coli.BL21

in which S100A6 can be induced to express by IPTG;Cell proliferation was measured by MTT

cell migration was measured by cell scratch assay and Transwell assay and levels of β-catenin were measured by ICC and Western-blotting.Results:① High purity recombinant S100A6(GST-S100A6) was obtained from E Coli via induction of IPTG induction.②Exogenetic S100A6 can not only inhibit 143B cell lines(positive control) proliferation but also the migration(P<0.05).However

effects of different concentration of S100A6 cast on proliferation(P>0.05) and migration(P>0.05) of these 5 transformed cell lines were not statistically significant.③ S100A6 can up-regulate β-catenin level in these 5 transformed cell lines significantly(P<0.05).Conclusion:Effects of S100A6 on these 5 transformed cell lines were not significant neither on proliferation nor on migration

but it can up-regulate their β-catenin levels.

关键词

S100A6转化细胞系细胞增殖细胞迁移β-catenin

Keywords

S100A6Transformed cell linescell proliferationcell migrationβ-catenin

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