Objective:To elucidate the effect of osteogenic growth peptide (OGP) on proliferation and differentiation of human periodontal ligament cells on titanium disc in vitro. Methods:Human periodontal ligament cells were cultured located on titanium disc individually in medium contained various concentrations of OGP;and cell proliferation quantity were examined by MTT and alkaline phosphatase activity were examined by enzyme-linked immunosorbentassay. Results:In vitro

the addition of OGP in cultures resulted in an increase of cell proliferation and ALP activity(P<0.05). All OGP concentrations used (10-11~10-7mol/L)were effective but the maximum response was obtained with 10-9mol/L OGP. Conclusion:Our results demonstrate that OGP stimulates proliferation and ALP expression of human periodontal ligament cells located on titanium disc in vitro and suggest that OGP is a promising growth factor in the region of oral implant.