高糖对肾小管上皮细胞Toll样受体4表达及其信号通路的影响

李曼丽; 甘华

doi:10.13406/j.cnki.cyxb.2010.06.023

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高糖对肾小管上皮细胞Toll样受体4表达及其信号通路的影响

基础研究 | 更新时间：2024-01-17

- 高糖对肾小管上皮细胞Toll样受体4表达及其信号通路的影响
- Effect of high glucose on the expression of Toll-like receptor 4 and the signal pathway in renal tubular epithelial cells
- 重庆医科大学学报 2010年35卷第6期页码：857-859
- 作者机构：
  
  重庆医科大学附属第一医院肾内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.06.023
  中图分类号： R587.24
- 纸质出版日期：2010，
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[1]李曼丽,甘华.高糖对肾小管上皮细胞Toll样受体4表达及其信号通路的影响[J].重庆医科大学学报,2010,35(06):857-859.

LI Man-li, et al. Effect of high glucose on the expression of Toll-like receptor 4 and the signal pathway in renal tubular epithelial cells[J]. Journal of Chongqing Medical University, 2010,35(6):857-859.
[1]李曼丽,甘华.高糖对肾小管上皮细胞Toll样受体4表达及其信号通路的影响[J].重庆医科大学学报,2010,35(06):857-859. DOI： 10.13406/j.cnki.cyxb.2010.06.023.

LI Man-li, et al. Effect of high glucose on the expression of Toll-like receptor 4 and the signal pathway in renal tubular epithelial cells[J]. Journal of Chongqing Medical University, 2010,35(6):857-859. DOI： 10.13406/j.cnki.cyxb.2010.06.023.

摘要

目的:通过研究高糖对肾小管上皮细胞Toll样受体4（Toll-like receptor4

TLR4）表达及其信号通路的影响

初步探讨TLR4与高糖促进肾脏细胞分泌炎症因子的关系。方法:小鼠近曲肾小管上皮细胞（MCT细胞）在高糖环境下培养后

用Western Blot分析TLR4蛋白的表达

Western Blot和免疫沉淀法分析TLR4与髓样细胞分化因子88（Myeloid differentiation factor 88

MyD88）的相互作用

以了解TLR4信号通路是否被激活。结果:高糖可使MCT细胞TLR4蛋白的表达上调

高糖环境下MyD88依赖的TLR4信号通路被激活。结论:TLR4与高糖促进肾脏细胞分泌炎症因子有关

TLR4参与糖尿病肾病的发病。

Abstract

Objective:To explore the role of Toll-likereceptor 4 in the production of inflammatory factors in renal innate cells stimulated with high glucose by studying the effect of high glucose on the expression of TLR4 and its signal pathway. Methods:Western Blot analysis was taken to measure the expression of TLR4

and the combination of Western Blot and immunoprecipitation was used to analyze the correlation of TLR4 with MyD88. Results:High glucose upregulated the expression of TLR4 protein and activated MyD88-dependent pathyway in MCT cells. Conclusion:TLR4 is involved in the pathogenesis of diabetic nephropathy by playing a role in the production of inflammatory factors in renal innate cells stimulated with high glucose.

关键词

Toll样受体4高葡萄糖肾小管上皮细胞

Keywords

toll-like receptor 4High glucoseProximal tubular epithelial cell

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