Objective:To explore the immunological competence of HBsAg-DCs vaccination prepared via HBsAg inducing DCs. Methods:The bone marrow cells of Lewis rat were cultured by RPMI1640 added with 4 ng/ml of granulocyte macrophage colony stimulating factor(GM-CSF)and 8 ng/ml of interlcukin-4(IL-4). 7 days late

the RPMI1640 were added with HBsAg(100 μg/ml)for another 48h to obtain HBsAg-DCs. The morphologic changes of HBsAg-DCs were observed by light microscope and the phaenotype change were detected by flow cytometry (FCM);The immuno-competence of the HBsAg-DCs were judge by one-way mixed lymphocyte reaction (MLR). Results:There were many DCs growing adherence in cluster and the suspended cells were obviously increasing and much bigger after administrating HBsAg. Compared with DCs

there were significant difference in T cell proliferation stimulated by HBsAg-DCs(P <0.05)

however

there was no significant difference in the T cell function. DCs inducing by HBsAg for 3 d could induce much more fortis. After treated by mitomycin C

T cell proliferation could be enhanced by DCs and HBsAg-DCs (P < 0.01)

and HBsAg-DCs were much more effective than the same density of DCs (P<0.01). Conclusion:The DCs loading with HBsAg have strong ability in presenting antigen.