鞘内靶向沉默5-HT<sub>5A</sub>受体基因对化疗痛的影响

闵卫翔; 刘巍; 刘瑜; 徐颖; 叶茂

doi:10.13406/j.cnki.cyxb.2010.05.041

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鞘内靶向沉默5-HT_5A受体基因对化疗痛的影响

基础研究 | 更新时间：2024-01-17

- 鞘内靶向沉默5-HT_5A受体基因对化疗痛的影响
- Effect of intrathecal down-regulation of 5-HT_5A receptors by RNA interference in rats with chemotherapy-evoked hyperalgesia
- 重庆医科大学学报 2010年35卷第5期页码：657-660
- 作者机构：
  
  重庆医科大学儿童医院麻醉科
- 作者简介：
- 基金信息：
  
  重庆市科委自然科学基金（编号:CSTC,2007BB5306）
- DOI：10.13406/j.cnki.cyxb.2010.05.041
  中图分类号： R730.5
- 纸质出版日期：2010，
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[1]闵卫翔,刘巍,刘瑜,徐颖,叶茂.鞘内靶向沉默5-HT_(5A)受体基因对化疗痛的影响[J].重庆医科大学学报,2010,35(05):657-660.

MIN Wei-xiang, et al. Effect of intrathecal down-regulation of 5-HT_5A receptors by RNA interference in rats with chemotherapy-evoked hyperalgesia[J]. Journal of Chongqing Medical University, 2010,35(5):657-660.
[1]闵卫翔,刘巍,刘瑜,徐颖,叶茂.鞘内靶向沉默5-HT_(5A)受体基因对化疗痛的影响[J].重庆医科大学学报,2010,35(05):657-660. DOI： 10.13406/j.cnki.cyxb.2010.05.041.

MIN Wei-xiang, et al. Effect of intrathecal down-regulation of 5-HT_5A receptors by RNA interference in rats with chemotherapy-evoked hyperalgesia[J]. Journal of Chongqing Medical University, 2010,35(5):657-660. DOI： 10.13406/j.cnki.cyxb.2010.05.041.

摘要

目的:观察鞘内注射5-羟色胺5A受体（5-hydroxytrptamine 5A receptor

5-HT5AR）的小干扰RNA重组腺病毒载体（Ad-5-HT5A-siRNA）前后

化疗痛大鼠脊髓5-HT5AR表达和痛觉过敏的变化情况

探讨5-HT5AR在化疗痛中的作用。方法:第一部分雄性成年SD大鼠随机分为模型组（n=10）和对照组（n=10）

模型组腹腔注射长春新碱

对照组腹腔注射生理盐水

定期测定大鼠的机械痛阈及RT-PCR检测建模结束时大鼠脊髓L4、5段5-HT5AR的mRNA表达。第二部分30只已成功建立化疗痛模型大鼠随机分为3组（n=10）

实验组鞘内注射Ad-5-HT5A-siRNA

阴性对照组鞘内注射阴性对照腺病毒（Ad-X-HK）

空白对照组鞘内注射生理盐水。鞘内注射后3、7d检测各组脊髓L4、5段5-HT5AR的表达

并分别在鞘内注射前及注射后3、5、7d测定机械痛阈。结果:腹腔注射长春新碱可引起模型组大鼠机械痛阈下降（P<0.01）和脊髓5-HT5AR的mRNA表达增高。而鞘内注射Ad-5-HT5A-siRNA后7d

实验组大鼠5-HT5AR的mRNA表达下降

同时该组大鼠的机械痛阈降低（与阴性对照组、空白对照组比较

P<0.05）。结论:5-HT5AR参与了化疗痛的调控过程

其表达增强时可能有减轻化疗痛的作用。

Abstract

Objective:To investigate the role of 5-HT5A receptors in rats with chemo the rapy-evoked hyperalgesia by intra the calinjection of adenovirus-mediated small-interference RNA.Methods:Adult male SD rats were randomly divided into 2 groups:model group(n=10

intraperitoneal injection of vincristine)and control group(n=10

intraperitoneal injection of normal saline).The mechanical hyperalgesia were measured as the paw withdrawal thresholds(PWT)and the expression of 5-HT5AR mRNA in L4、5 spinal cord were determined by RT-PCR.Thirty rats with chemotherapy-evoked hyperalgesia were randomly divided into 3 groups after the formation of chemotherapy pain model:experimental group

negative control group and blank control group

which respectively were treated with intrathecal injection of Ad-5-HT5AR-siRNA

Ad-X-HK and normal saline.Expressions of 5-HT5AR mRNA and PWT after intrathecal injection were assessed respectively.Results:Compared with control group

PWT of rats in model group were significantly decreased(P<0.01)

and levels of 5-HT5AR mRNA were increased.But after intrathecal injection

expressions of 5-HT5AR mRNA were down-regulated in experimental group.Meanwhile

PWT of rats in experimental group were lower than those in negative control group and blank control group(P<0.05).Conclusion:5-HT5AR is involved in the mechanism of chemotherapy-evoked hyperalgesia and may has antinociceptive effect.

关键词

5-HT5A受体化学治疗神经病理性疼痛鞘内注射RNA干扰

Keywords

5-HT5 AreceptorchemotherapyNeuropathic painIntrathecal injectionRNA interference

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鞘内靶向沉默5-HT5A受体基因对化疗痛的影响

Effect of intrathecal down-regulation of 5-HT5A receptors by RNA interference in rats with chemotherapy-evoked hyperalgesia

DOI：10.13406/j.cnki.cyxb.2010.05.041

摘要

Abstract

关键词

Keywords

references

鞘内靶向沉默5-HT_5A受体基因对化疗痛的影响

Effect of intrathecal down-regulation of 5-HT_5A receptors by RNA interference in rats with chemotherapy-evoked hyperalgesia