Objective:Study on establishing HA animal model

which isoperated simply and have short model building period and high successful rate

to provide favorable test materials for HA-related scientific research.Methods:10 SD rats were randomly selected to set up the model by using APH intraperitoneal injection

meanwhile rats intraperitoneally infused with saline served as controls group.After 8 days of experimental treatment

we evaluated the animal model by using blood analysis

reticular count

myelogram and other laboratory experimental results.Results:On eighth day after experimental treat

the results of RBC、HGB、PLT、Ret% in Rats’peripheral blood are respectively(2.98±0.623)×1012/L、(100±14.2)g/L、(320±358.8)×109/L、90.7%±4.23%

the concentration of indirect bilirubin and free-haemoglobin in plasma are respectively(10.3±3.30)μmol/L、(1 248±282.1)mg/L.Compared to the control group

the difference of the results all have the statistical significance;bone marrow exhibited that karyocytes proliferate obviously

mainly the erythroid system.Conclusion:APH intraperitoneal injection can set up HA animal model shortly and conveniently

and the animal model has the clinical features.So the method of APH intraperitoneal injection is an ideal way to establish HA animal model