Objective:To discuss the different expression of AQP8 in cultured normal human trophocyte and JAR cell line

and the relationship between the changed expression of the channel protein and nosogenesis of chorionic carcinoma.Methods:The human cultured normal trophocyte was primarily cultured

purified and identified.JAR cell line was cultured.The expression of AQP8 was detected in cultured normal human trophocyte and JAR cell line by immunohistochemistry immunofluorescence confocal laser and RT-PCR.Results:(1)In the control group

the limitied expression of AQP8 was observed in cultured normal human trophocyte and JAR cell line

but in the exprimental group

the rate of positive cell of AQP8 in JAR cell line were 93.27%±14.45%

much higher than that in the control group.(2)By immunofluorescence staining confocal laser imaging

it was revealed that

in the same condition and excitation intensity

AQP8 in the JAR choriocarcinoma cell lines showed positive expression of the green

while in normal trophoblast cells

no green light

but when the excitation intensity was strengthened

it may still explore AQP8 in normal human villous trophoblast cell expression(.3)The expression of AQP8mRNA was detected in both cultured normal human trophocyte and JAR cell line.The OD value(0.98±0.35)of AQP8 in the experimental group was significantly higher than that(0.32±0.04)in the control group.Conclusion:Abnormal overexpression of AQP8 will destroy the external water and electrolyte balance of trophoblast cells

which might be one of the reasons for the occurrence of choriocarcinoma.