Objectives:To explorer the influence of siRNA-Smad3-mediated Smad3 gene silence on the expression of fibronectin and collagenⅠin human keloid fibroblast(sKFB).Methods:One specific Smad3-siRNA fragment and another irrelevant siRNA fragment was respectively transfected into human primary KFB cells.RT-PCR

Western blot

immunohistochemisty and immunofluorescence were employed to evaluate the changes in either the expression or the secretion of both fibronectin and collagenⅠ.Restults:The expression and secretion of both the fibronectin and collagenⅠin KFB were inhibited significantly by the treatment of Smad3-siRNA for 48 hours at a final concentration of 75nmol/L as compared with those of the untreated or the irrelevant siRNA fragment treated group.Conculsion:Smad3-siRNA could be employed to specifically inhibit the Smad3 gene expression in KFB cells and to efficiently inhibit both the synthesis and secretion of fibronectin and collagenⅠ.