Objective:To dynamically observe changes of Th1/Th2 type immune responses in mice immunized with recombinant Bifidobacteria bifidum(Bb)-Eg95-EgA31 vaccine of Echinococcus granulosus(Eg).Methods:BALB/c mice were vaccinated by 5×108 colony forming unit(CFU) orally and 5×105CFU intranasally respectively.Mice were killed on week 2

4

6

8

10

12

14

16

18 and 20 after immunization respectively

sera were collected to measure the levels of IgG

its subclasses and IgE by enzyme linked immunosorbent assay(ELISA);Spleens were removed to determine the levels of IFN-γ

IL-12

TNF-αand IL-10 of splenocyte culture supernatant by ELISA.Results:Oral immunization mice showed higher levels of IgG

IgG2a

IgG2b

IgG1

IgG3 and IgE on the 8～10th week

2～20th week

2～20th week

4～8th week

6～12th week and 10th week respectively

while IFN-γ

IL-12

TNF-αand IL-10 on the 2～16th week

2～12th week

2～6th week and 4～12th week post vaccination respectively;Intranasal immunization mice showed higher levels of IgG

IgG2a

IgG2b

IgG1

IgG3 and IgE during the 4～10th week

4～20th week

2～20th week

2～12th week

4～12th week and 10～12th week respectively

while IFN-γ

IL-12

TNF-αand IL-10 on the 2～8th week

2～12th week

2～8th week and 6～16th week post vaccination respectively.Conclusion:Mixed Th1/Th2 type immune responses may be induced in mice immunized with the recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus.Oral and intranasal inoculation are two better immune routes

and the former is better than the latter.