Objective:To study the triglyceride level changes with or without Diammounium Glycyrrhizinate(DIG)and the potential mechanism on alcoholic fatty liver cell model induced in vitro.Methods:MTT assay was used to screen the optimal concentration of ethanol and DIG injection.Ultra-microstructure was observed under transmission electron microscope.Intracellular triglyceride concentration was measured by hol-automatic biochemistry.Expressions of peroxisome proliferator-activated receptor-gamma (PPARγ)

sterol regulatory element binding protein-1(SREBP-1)and SREBP cleavage activating protein(SCAP)were determined by immunocyto-chemistry(ICC).Results:1.The screened optimal DIG concentration was 2.273μg/ml.2.Masses of lipid droplets in cytoplasm were observed under transmission electron microscope.3.Hol-automatic biochemistry measured intracellular triglyceride concentration to be(1.84±0.16)mmol/L and(1.05±0.12)mmol/L respectively in Ld30 group and DIG group

and the difference was statistically significant(P<0.01).4.The expression of PPARγ

SREBP-1

and SCAP in DIG group decreased

as compared with Ld30 group

and the difference was statistically significant.Conclusion:DIG is helpful to relieve steatosis of alcoholic fatty liver induced in vitro

and its possible mechanism is by down-regulating expressions of PPARγ

SREBP-1

and SCAP

thus lipogensis is inhibited in the liver cells.