NHE1反义基因对胃癌细胞NHE1蛋白表达及裸鼠成瘤力的影响

滕小春; 刘海峰

doi:10.13406/j.cnki.cyxb.2010.01.042

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NHE1反义基因对胃癌细胞NHE1蛋白表达及裸鼠成瘤力的影响

基础研究 | 更新时间：2024-01-17

- NHE1反义基因对胃癌细胞NHE1蛋白表达及裸鼠成瘤力的影响
- Effect of Antisense gene NHE1 on the expression of NHE1 protein of gastric cancer cell after transfection
- 重庆医科大学学报 2010年35卷第1期页码：57-59
- 作者机构：
  
  1. 重钢总医院老年科
  2. 北京武警总医院消化科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.01.042
  中图分类号： R735.2
- 纸质出版日期：2010，
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[1]滕小春,刘海峰.NHE1反义基因对胃癌细胞NHE1蛋白表达及裸鼠成瘤力的影响[J].重庆医科大学学报,2010,35(01):57-59.

TENG Xiao-chun, et al. Effect of Antisense gene NHE1 on the expression of NHE1 protein of gastric cancer cell after transfection[J]. Journal of Chongqing Medical University, 2010,35(1):57-59.
[1]滕小春,刘海峰.NHE1反义基因对胃癌细胞NHE1蛋白表达及裸鼠成瘤力的影响[J].重庆医科大学学报,2010,35(01):57-59. DOI： 10.13406/j.cnki.cyxb.2010.01.042.

TENG Xiao-chun, et al. Effect of Antisense gene NHE1 on the expression of NHE1 protein of gastric cancer cell after transfection[J]. Journal of Chongqing Medical University, 2010,35(1):57-59. DOI： 10.13406/j.cnki.cyxb.2010.01.042.

摘要

目的:探讨人NHE1反义基因转染对SGC-7901胃癌细胞NHE1蛋白表达及裸鼠成瘤力的影响。方法:构建人NHE1反义基因真核表达载体

采用脂质体法将其转染至SGC-7901胃癌细胞中

通过免疫组织细胞化学SP法检测NHE1蛋白表达情况

流式细胞仪检测对细胞增殖和凋亡的影响

从电镜水平比较观察转染与未转染细胞形态学的变化

并观察裸鼠成瘤能力的改变。结果:转染NHE1反义基因的SGC-7901胃癌细胞NHE1蛋白表达降低;细胞增殖能力下降

细胞凋亡率增加;透射电镜下细胞线粒体数量增多、体积增大

内质网扩张

核偏向一侧

极性增强;裸鼠成瘤能力下降。结论:NHE1反义基因能使SGC-7901胃癌细胞的NHE1蛋白表达明显下调

裸鼠成瘤能力降低

NHE1蛋白在胃癌细胞生长中起重要作用。

Abstract

Objective:To study the effects ofantisense human NHE1 gene on the expression ofthe NHE1 protein ofSGC-7901 gastric cancer cell and the changes of the carcinoma forming capacity after transplantation in nude mouse.Methods Antisense NHE1 eukaryotic expression vector was constructed with recombinant DNA technique and transfected into the cells of gastric carcinoma cell line SGC-7901 with liposome DOTAP.Then

the expression of NHE1 protein with SP and the changes of the carcinoma forming capacity in nude mouse was also observed.Results:The expressions of NHE1 protein of SGC-7901 cells transfected with antisense NHE1 gene in vitro were obviously decreased

inhibitions of their proliferation were induced

apoptosis rate increased.Under electron microscope

organelles in these cells were abundant.carcinoma forming capacity in nude mouse were decreased.Conclusion:The expression of NHE1 protein was obviously down regulated after the transfection of antisense NHE1 gene

and carcinoma forming capacity in nude mouse was decreased.which implies that NHE1 protein plays an important role in gastric cancer cell growth.

关键词

NHE1基因真核表达载体反义基因胃癌

Keywords

NHE1 geneeukaryotic expression vectorAntisense genegastric cancer

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