LU Wei, et al. Comparison of two methods to purfy rat follicle stem cell[J]. Journal of Chongqing Medical University, 2010,35(1):124-126. DOI: 10.13406/j.cnki.cyxb.2010.01.002.
Objective:To compare the advantage and disadvantage of two methed purfy rat follicle stem cells by microdissection and Vario magnetic activated cell sorting(MACS) or flowcytometry in combination.in order to further explore the rat follicle stem cells in vitro culture conditions.Methods:rat follicle bulg digested into cell suspension after obtained by microdissection.Observation of cell morphology and to detect cells before and after sorting the purity
activity
recovery
identification by immunofluorescence.Results:All Two methods can be purfy rat follicle stem cell.By flow cytometry sorting cells with high purity and low activity than MACS sorting cell.Conclusion:flow cytometry and MACS both can effectively obtain the hair follicle stem cells.Microdissection combine which method depend on the experimental requirements.