Objective:To investigate the effect of RPM on bcl-2 and Fas protein expressions at different ischemia-reperfusion points.Methods:Acute ischemic renal injury model was established(right kidney ectomized

45 min of left renal ischemia and reperfusion for Oh

24h

48h and 72h).Fifty four normal male Wistar rats were.randomly divided into 3 groups:sham-operated group(sham

without renal ischemia)

ischemia-reperfusion group(IR

without RPM)

RPM and ischemia-reperfusion group(RPM 4mg/(kg·d)×3d

reperfusion at 2h before

operation and each observation day).Pathomorphological changes of renal ischemia- reperfusion injury were observed by HE stain and SABC immunohistochemical methods were used to detect the changes of expressions of bcl-2 and Fas.Results:bcl-2 and Fas concentration were significantly higher in IR group than those in sham group(P<0.05).As compared with ischemia-reperfusion group(P<0.05)

bcl-2 expression increased and Fas expression decreased significantly in RPM+IR group.In IR group local necrosis of renal tubule epithelium cells was found by HE stain and large quantity of apoptototis residues were observed

while similar changes were scarcely visible in RPM+IR group. Conclusion:RPM has significant effect on the up-regulation of bcl-2 expression and down-regulation of Fas expression in renal ischemia-reperfusion injury to achieve the purpose of alleviating the injury.