吲哚美辛体外干预研究胃癌Wnt/β-Catenin信号途径与Caspase-3及Bcl-2关系

王颢; 魏莎莉; 杨戎; 刘孝云

doi:10.13406/j.cnki.cyxb.2008.03.023

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吲哚美辛体外干预研究胃癌Wnt/β-Catenin信号途径与Caspase-3及Bcl-2关系

论著 | 更新时间：2024-01-17

- 吲哚美辛体外干预研究胃癌Wnt/β-Catenin信号途径与Caspase-3及Bcl-2关系
- Reaserch of relationship between Wnt/β-Catenin signal path-way in gastric carcinoma and the expression of Caspase-3 and Bcl-2 induced in vitro by indomethici
- 重庆医科大学学报 2008年第3期页码：304-307
- 作者机构：
  
  1. 重庆医科大学公共卫生学院生殖生理教研室
  2. 重庆医科大学公共卫生学院生殖生理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.03.023
  中图分类号： R735.2
- 纸质出版日期：2008，
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[1]王颢,魏莎莉,杨戎,刘孝云.吲哚美辛体外干预研究胃癌Wnt/β-Catenin信号途径与Caspase-3及Bcl-2关系[J].重庆医科大学学报,2008(03):304-307.

WANG Hao. Reaserch of relationship between Wnt/β-Catenin signal path-way in gastric carcinoma and the expression of Caspase-3 and Bcl-2 induced in vitro by indomethici[J]. Journal of Chongqing Medical University, 2008,(3):304-307.
[1]王颢,魏莎莉,杨戎,刘孝云.吲哚美辛体外干预研究胃癌Wnt/β-Catenin信号途径与Caspase-3及Bcl-2关系[J].重庆医科大学学报,2008(03):304-307. DOI： 10.13406/j.cnki.cyxb.2008.03.023.

WANG Hao. Reaserch of relationship between Wnt/β-Catenin signal path-way in gastric carcinoma and the expression of Caspase-3 and Bcl-2 induced in vitro by indomethici[J]. Journal of Chongqing Medical University, 2008,(3):304-307. DOI： 10.13406/j.cnki.cyxb.2008.03.023.

摘要

目的:研究人胃癌细胞系SGC7901中β-连环蛋白（β-Catenin）与Caspase-3凋亡基因及与Bcl-2原癌基因表达的关系

初步探索肿瘤发生过程中Wnt/β-Catenin通路对细胞凋亡的调节机制。方法:（1）MTT方法检测吲哚美辛抗肿瘤效应;（2） RT-PCR分别检测人胃癌细胞系SGC7901受吲哚美辛干预组和未受干预组中的β-Catenin与Caspase-3及Bcl一2mRNA的表达。结果:吲哚美辛在浓度为50μmol/L时作用24h对人胃癌SGC7901细胞抑制效果不明显

而作用48、72h及浓度为100和200μmol/L时作用24、48、72h的凋亡诱导作用明显

且抑制率呈浓度和时间依赖性;吲哚美辛干预组较未受干预组表现为伴随β-Catenin表达水平降低

Bcl-2表达下调

而Caspase-3的表达上调。结论:人胃癌细胞系SGC7901可能通过Wnt/β-Catenin通路激活Bcl-2原癌基因蛋白表达促进细胞增殖

同时抑制Caspase-3凋亡基因的蛋白表达抑制细胞凋亡

使细咆增殖与凋亡失衡。

Abstract

Objective:To study the regulatory mechanism of Wnt/β-Catenin passageway influenced apoptosis of human gastric cancer line SGC-7901 by detecting the expression ofβ-Catenin mRNA

Caspase-3 mRNA and bcl-2 mRNA.Methods:(1) Methyl thiazolyl tetrazolium(MTT)assay was used to measure the anticancer activity of indomethaci.(2)Expression ofβ-Catenin

Caspase-3 mRNA and bcl-2 mRNA in groups of without induced or induced by indomethaci were detected by RT-PCR. Results:Indomethacin of 50μmol/L at 24h couldn’t induce apoptosis of gastric cancer cells.Indomethacin of 50μmol/L at 48 and 72h and of 100μmol/L and 200μmol/L at 24

48 and 72h could induce apoptosis of gastric cancer cells in adose- and time-dependent manner;Compared with groups of without induced by indomethaci

expression ofβ-Catenin mRNA and bcl-2 mRNA were lower

however

increased expression of Caspase-3 mRNA were found in SGC7901 cells after treatment with indomethaci.Conclusion:Apoptosisin inbalance may be caused by activating expression of bcl-2 proto-oncogene protein and inhibiting expression of Caspase-3 protein through Wnt/β-Catenin passageway in human gastric cancer line SGC7901.

关键词

胃癌细胞凋亡Wnt/β-Catenin通路

Keywords

gastric cancerapoptosisWnt/β-Catenin passageway

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