Objective:To explore the effect of angelica polysaccharide(APS)on adhesion molecules expressions in murine hematopoietic cells

in order to provide experimental evidence of APS on hematopoietic regulation and the mechanism of mobilizing.Methods:Techniques of peripheral blood white blood cell(WBC)and bone marrow mononuclear cells(MNC)count were made.Flow cytometry detected the rate of Sca-1~+ cells in peripheral blood and bone marrow and the expression of CD49d (VLA-4 of a chain)

CD44 on bone marrow MNC and Sca-1~+ cells.Results:1.In APS group(4mg/kg):the number of peripheral blood WBC and Sca-1~+ cells were markedly higher than that of NS group(P<0.01)

and bone marrow MNC and Sca-1~+ cells was significantly lower than that of NS group(P<0.01);2.In APS group:the expression of CD49d on bone marrow MNC and Sca-1~+ cells significantly declined(P<0.05);the percent of CD44’expression on bone marrow MNC also decreased(P<0.05)

but we did not observe any change of the mean fluorescence intensity at the same time

or that of CD44’expression on bone marrow Sca-1~+ cells.Conclusion:APS may reduce adhesion molecules expressions on some hematopoietic cells

resulting in mobilizing hematopoietic stem and progenitor cells cells(HSPC)from the bone marrow into the peripheral blood.