Objective:To explore the mechanism of early onset subclinical seizures following hypoxic brain injury. Methods:SD male adult rats were assigned randomly to control rats and rats exposed to global hypoxia by 8% oxygen nitrogen atmosphere. According to electroencephalogram recording seizure discharge

hypoxie rats were divided into subclinical seizures group and non-subclinical seizures group

then the changes in neuropathology and the expression of GFAP in cortex and hippocampus of rat brain were studied by Nissle-staining immunohisochemistry and western-blot. Results:Subclinical seizures occurred in 19.67% rats following hypoxia. Compared with non-subclinical seizure group

neuronal loss of hippocampal CA1 and CA3 region as wel as temporal cortex were distinct in subclinical seizure group(P<0.05). Meanwhile

the expresssion of GFAP in subclinical seizure group was stronger than that of non-subclinical seizure group

especially in hippoeampal area(P<0.05). Conclusion:Onset subclinical seizures occured following hypoxic injury in adult rats

which may be related to increased GFAP expression and neuronal loss following hypoxic brain damage.