Objective:To explore the correlation of NF-K B signal fractions and RCC onset and development

and influence of As

2

O

3

on NF-K B signal transduction in RCC 786-0 cell.Methods:NF-K B DNA binding activity

p65 mRNA

p65

IK- Kα/β

I K Bαand ICAM-1 protein were investigated in 15 RCC tissues and 10 normal renal tissues

and human RCC 786-0 cell by ICC

ISH

Western blot and EMSA.Results:In RCC tissues

NF-K B DNA binding activity

p65

IKKα/βand I- CAM-1 protein were increased

compared to normal renal tissues（P

<

0.01）

while concomitantly expression of I K Bαwas de- creased（P

<

0.01）;treatment with≥2μmol/L As

2

O

3

could effectively decrease expression of p65 mRNA

p65

IKKα/β

I K Bαand ICAM-1 protein（P

<

0.01）

and inhibit NF-K B DNA binding activity（P

<

0.01）.Conclusions:High NF-k B DNA binding activity

p65

IKKα/βand ICAM-1 protein expression may play an important role in onset and development of RCC;≥2μmol/L As

2

O

3

can effectively inhibit activation of NF-K B signal pathway in 786-0 cells

and it is suggested that As

2

O

3

can depress proliferation of RCC 786-0 cells by inactiving NF-K B signal transduction

and NF-K B might be a target of an- ti-RCC.