Study on of metallo-β-1actamases in pseudomonas aeruginosa[J]. Journal of Chongqing Medical University, 2007,(5):497-499. DOI: 10.13406/j.cnki.cyxb.2007.05.007.
Objective:To monitor the rug resistance and epidemiology state of pseudomonas aeruginosa(PA)isolated clinical pa- tient.Methods:antimicrobial sensitivity tests were performed by K-B method and metallo-β-lactamases-producing PA was genotyped by the randomly amplified polymorphic(PAPD)assay.Results:Among24 strains resistant to imipenem.19(8%)strains produced metal enzyme which could be distingurished into 10 kinds of PAPD type.the type ability was 100%.There was same gene type strain.Isolated from three patient and there were two kinds of gene types. Among 2 strains isolated from the same patient at the different time.Conclusions:the K-B method could be used to determine metal enzyme
rapidly and eonviently. And PAPD could to be used in the molecular epidemiological study of PA.