Objective:To construct the eukaryotic recombinant bacmid containing fusion protein gene of human metapneumovirus and express it in insect cells.Methods:The full-length fusion protein gene of human metapneumovirus was amplified by re- verse transcriptional polymerase chain reaction(RT-PCR).After inserted at the multiple clone site of donor plasmid

pFast- BacHT

target gene was delivered to bacmid to yield a recombinant bacmid.This recombinant baemid was subsequently pack- aged in sf-9 cells and infectious recombinant baculovirus was used to inoculate fresh sf-9 cells to express fusion protein.Ex- pressed protein was purified by using a 6xHis tag rasin and identified by western bloning.Result:Nucleotide sequence analy- sis of PCR products amplified from rBacmid-hMPVF showed 100% identity to public sequence of CAN 97-83 F gene se- quence.Sf-9 cells infected by recombinant baculovirus displayed typical cytopathic effects.By using 6xHis tag monoclonal an- tibody and western blotting

a single band with molecular weight around 70ku was identified.Conclusion:The fusion protein of hMPV is successfully expressed by using baculovirus expression system in insect cells

which will benefit the study on sero-epidemiology and pathogenesis of hMPV infection in the future.