objective: To examine the potential of ANP63 and HPV16 E7 as one of biomarkers for cervical premalignant lesions in Thi-nPrep smears

and to search for the identification of specific biomarkers for cervical intraepithelial neoplasia (CIN) as a new examining method to evaluate evolution of CIN. Method; Streptavidin peroxidase (SP) method was used. Immunocytochemical analysis of ANP63 expression was performed in 71 ThinPrep smears. RT - PCR was used to detect the mRNA of HPV16 E7 in 71 ThinPrep liquid - based smears specimens. Result; Positive expression rate of ANP63 in CIN was higher than that in inflammation (P <0.01). The positive expression rate of HPV16 E7 in CIN was higher than that in inflammation (P < 0.01). Conclusion; Overexpression of ANP63 can be a useful marker to reduce false - negative cytology interpretation. ANP63 should show indirectly the activation of HPV16 E7

which should be beneficial to evaluate the tumorigensis of HPV 16.