Objective;To investigate whether the traditional Chinese medicine Yisheng Injection can reduce the injury on isolated rat heart induced by cold ischemia. Methods ;18 isolated female closed colony SD rats were divided into two groups at random. In the control group

the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃

while in the experimental group

the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection at 4℃. The specimens were harvested 2

6

12

24

48 and 72 hours after preservation. Histochemical changes were observed in two groups. Results ;In the control group

the activities of alkaline phosphatase ( ALP) and NADPH diaphorase (NADPHD) decreased after cold ischemia for 24 hours. Lactic dehydro-genase (LDH) decreased slightly

while ALP and NADPHD decreased evidently

after cold ischemia for 48 hours. Succinic dehydro-genase ( SDH) and cytochrome oxidase ( CCO) decreased slightly

and NADPHD disappeared after cold ischemia for 72 hours respectively. In the experimental group

NADPHD

LDH and CCO decreased slightly after cold ischemia for 24

48 and 72 hours. ALP decreased slightly after cold ischemia for 48 hours

however

SDH showed no changes

after cold ischemia for 72 hours. Conclusion : Cold ischemia can induce cold ischemia injury in isolated rat hearts

especially the vascular endothelial cells being more sensitive. Yisheng injection can reduce this kind of injury to some degree.