Objective:To establish and assess a modified mouse asthma model.Methods:Clean female BALB/c mice were divided into 2 groups(n20

10 for each group).The asthmatic group was sensitized by i.p.and i.n.with OVA aluminum potassium sulfate solution on day 0 and 14.Mice were challenged via the airways with OVA(5% in saline) for 14 days (from day 21 to 35) using ultrasonic nebulization.The control group received saline as the substitution of OVA.Results:The asthmatic group demonstrated the symptoms of acute asthma

such as scratching the head continuously

breathing deeply and fast

staying still

bowing the back

lifting the forelimbs

and so on.There were more leukocytes and eosinophils in the BALF of asthmatic group.In either blood or BALF

the levels of IL-4 and OVA specific IgE were significantly elevated while the IFN-γ level was lower in the asthmatic group

as compared with the control group.In asthmatic mice

inflammatory cell increased.The goblet cell hyperplasia

smooth muscle cell proliferation and pulmonary emphysema were much more serious in asthmatic mice than in control mice.Conclusion:This successful modified mouse asthma model may provide a necessary tool in the fundamental research of asthma.