CHENG Qingfeng, et al. Establishment of Botnia clamp technique in healthy women[J]. Journal of Chongqing Medical University, 2005,(4):598-600. DOI: 10.13406/j.cnki.cyxb.2005.04.032.
Objective:To establish a method for independent assessment of insulin secretion and insulin sensitivity (SI) simultaneously.Methods:B-cell function and insulin sensitivity in 13 healthy feminine subjects were assessed by applying intravenous glucose tolerance test combined with hyperinsulinemic-euglycemic-clamp and the test was compared with regular hyperinsulinemic- euglycemic-clamp.Results:There was no signal statistical difference when campared the stable (150~180 min)M-value obtained during the Botnia clamp with the stable M-value from a regular hyperinsulinemic- euglycemic-clamp (13.11±1.71mg/(kg·min)
13.34±1.41mg/(kg·min)
respectively
P>0.05).Stable glucose concentrations were (5.14±0.12)mmol/L and(5.15±0.14)mmol/L
respectively
P>0.05.Conclusion:The prior glucose bolus does not affect the following euglycemic clamp.The Botnia clamp is a useful method for simultaneous independent assessment of insulin secretion and insulin sensitivity.