CHENG Chaoying, et al. Resistance of different methods of hemoglobin assay to common interference[J]. Journal of Chongqing Medical University, 2005,(4):591-593.
CHENG Chaoying, et al. Resistance of different methods of hemoglobin assay to common interference[J]. Journal of Chongqing Medical University, 2005,(4):591-593. DOI: 10.13406/j.cnki.cyxb.2005.04.030.
Objective:To investigate the effects of common inhibitors of the antioxidation system and vitamin C on the measurement of hemoglobin (Hb).Methods:Hemolyzed rabbit red blood cell was used as the sample.The interferences of common inhibitors of the antioxidation system on assay of Hb by its absorbance at 540nm
alkalinized Hb by absorbance at 575nm
the azide-Hb complex by absorbance at 542nm were compared.Results:Vitamin C and the inhibitors of superoxide dismutase
catalase exhibited larger interferences with direct assay of Hb by absorbance at 540 nm and the assay of azide-Hb
and inhibitor of GSH and GSH peroxidase showed smaller effects.The assay of alkalinized Hb exhibited the strongest resistance to all these chemicals.Conclusion:The assay of alkalinized Hb shows the strongest resistance to common chemicals used to investigate the mechanism of peroxidative damage and can be applicable to the study of the mechanism of vitamin C-induced hemolysis.