Objective:To explore the synergism of vascular endothelial cells and fibroblasts derived from tumors in the tumor angiogenesis after the establishment of the cell models.Methods:Reverse transcription polymerase chain reaction

two-well co-culture system were used to detect the tolomerase activity

and their abilities of migration and angiogenesis of ECV304-SKOV3 cells and the potential of L

929

-H

22

to promote angiogenesis and invasion of MDA-MB-231 cells

respectively

which were established as the cell models for vascular endothelial cells and fibroblasts derived from tumors in our laboratory before.Results:Tolomerase activity level of ECV304-SKOV3 cells was higher than that of ECV304 cells （0.778±0.011 vs 0.692±0.014

P

<

0.001）

so were L

929

-H

22

cells and L

929

cells （0.875±0.026 vs 0.684±0.012

P

<

0.001）.The migratory capability of ECV304-SKOV3 cells was 3.10 times that of ECV304 cells（P

<

0.001）.ECV304-SKOV3 cells formed tubular structures more and longer than ECV304 cells did when co-cultured with SKOV3 cells（P

<

0.01）.L

929

-H

22

and L

929

cell lines enhanced the invasiveness of human mammary cancer MDA-MB-231 cells through artificial basement membrane （Matrigel） 1.21 and 0.48 respectively（P

<

0.001）.Fibroblasts L

929

-H

22

stimulated endothelial cells to form more and longer tubes than L929 did（P

<

0.05）

especially when L

929

-H

22

and ECV304-SKOV3 cells were co-cultured.Conclusion:The results suggest that vascular endothelial cells and fibroblasts derived from tumors survive longer with greater ability to migrate and form tubes

and promote tumor cells invasion and vascular endothelial cells to form tubes.The two kinds of cells have a synergic role in the tumor angiogenesis.