Objective:To study the effect of polycystic ovary-like environment on secretion of E

2

and P from human granulosa luteal cells in vitro.Methods:According to different additives granulosa cells cultured in vitro which were retrieved from infertility women with normal ovulation who were undergoing in vitro fertilization-embryo transfer（IVF-ET）

were divided into four groups:A （androstenedione） group

LH group

INS （insulin） group and A+LH+INS group.Each group was subdivided into polycystic ovary-like environment group with higher levels of the additives and physiological environment group with lower levels of the additives.Levels of E

2

and P in medium were detected separately.Results:Levels of E

2

and P in polycystic ovary-like environment were significantly decreased in A group and A+LH+INS group

and significantly increased in LH group and INS group （P

<

0.01 respectively）.Levels of E

2

and P of LH group

INS group and A+LH+INS group were all significantly higher than that of A group

with the highest level of E

2

in INS group and the highest level of P in LH group.Moreover

compared with INS group or LH group respectively

concentrations of E

2

and P in A+LH+INS group were both significantly decreased in polycystic ovary-like environment but no difference was found in physiological environment.Conclusion:In polycystic ovary-like environment

E

2

and P secretion from normal granulosa luteal cells may be inhibited by A.