双重酶标法检测溃疡性结肠炎凋亡上皮细胞Fas的表达

严瑾; 朱玉娟; 黄丽彬

doi:10.13406/j.cnki.cyxb.2005.04.007

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双重酶标法检测溃疡性结肠炎凋亡上皮细胞Fas的表达

更新时间：2024-01-17

- 双重酶标法检测溃疡性结肠炎凋亡上皮细胞Fas的表达
- Detection of Fas on colonic apoptosis epithelium in ulcerative colitis by double staining immunohistochemistry
- 重庆医科大学学报 2005年第4期页码：509-512
- 作者机构：
  
  1. 四川大学华西医院消化科
  2. 成都363医院
  3. 四川大学华西医院消化科,成都,610041
- 作者简介：
- 基金信息：
  
  四川省科技厅重点科技项目（02sy029-112）
- DOI：10.13406/j.cnki.cyxb.2005.04.007
  中图分类号： R574.62
- 纸质出版日期：2005，
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[1]严瑾,朱玉娟,黄丽彬.双重酶标法检测溃疡性结肠炎凋亡上皮细胞Fas的表达[J].重庆医科大学学报,2005(04):509-512.

YAN Jin, et al. Detection of Fas on colonic apoptosis epithelium in ulcerative colitis by double staining immunohistochemistry[J]. Journal of Chongqing Medical University, 2005,(4):509-512.
[1]严瑾,朱玉娟,黄丽彬.双重酶标法检测溃疡性结肠炎凋亡上皮细胞Fas的表达[J].重庆医科大学学报,2005(04):509-512. DOI： 10.13406/j.cnki.cyxb.2005.04.007.

YAN Jin, et al. Detection of Fas on colonic apoptosis epithelium in ulcerative colitis by double staining immunohistochemistry[J]. Journal of Chongqing Medical University, 2005,(4):509-512. DOI： 10.13406/j.cnki.cyxb.2005.04.007.

摘要

目的:探讨Fas/FasL介导的结肠上皮细胞凋亡在溃疡性结肠炎（UC）发病机制中的作用。方法:明确诊断的35例UC病人和15例对照人群的活检标本分别进行Fas、FasL的免疫组化染色

采用M30 Cytodeath特异性地检测结肠上皮细胞早期凋亡;对Fas及M30的表达进行相关性分析。观察连续切片和双重酶标染色中Fas和M30的共表达关系。结果:UC组上皮细胞表达Fas、FasL及M30的水平均比对照组明显增高（P<0.01）;Fas与M30的表达有相关性（P<0.05）;通过连续切片分别染色Fas与M30

以及双重酶标法同时染色Fas与M30

发现UC病变肠道凋亡的上皮细胞均为Fas表达阳性的细胞。结论:Fas/FasL介导的结肠上皮细胞过度凋亡可能是UC上皮层破坏的机制之一。

Abstract

Objective:To discuss the role of Fas/FasL-mediated colonic epithelial apoptosis in the pathogenesis of ulcerative colitis(UC).Methods:Expression of Fas

FasL and M30(a new epithelium apoptosis marker) antigen on colonic mucosa of 35 patients with UC and 15 control populations were detected by IHC.Each marker was analyzed by image analysis system.Co-expression of Fas and M30 was observed through serial sections and double staining IHC.Results:The expression of Fas

FasL as well as M30 in UC increased considerably compared with controls (P<0.01).There was correlation between expressions of Fas and M30 in UC.Fas and M30 antigen coexpression was found in sequence slices and double staining IHC slices.Conclusion:Fas/FasL-mediated colonic epithelial apoptosis may be one of major causes which lead to damage of the epithelial barrier.

关键词

溃疡性结肠炎双标染色Fas凋亡

Keywords

ulcerative colitisDouble staining IHCFasapoptosis

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