Objective:To investigate the sensibility of different methods to test MRCNS

and analyze the antibiotic resistance of CNS.Methods:200 strains CNS were detected by mecA genic

E-test

PBP2a latex agglutination

OXA agar election and K-B

and analyze the resistance with WHONET4.Results:The positive rates of MRCNS by agar election method

K-B

E-test

PBP2a latex agglutination

mecA genic were 70%

74%

72%

71%

73.5%respectively.There is no statistic significance in 5 assays(χ2=1.5124

P>0.5).The resistance rates of MRCNS to PEN

GEN

SXT

CIP

TET

ALL and ERY

are all above 50%.The resistant rates of MSCNS to other antibiotics are below about 50% except for PEN(80.4%)and ERY(57.1%).MRS are more than 70% and no VAN resistant in CNS.Conclusion:All the 5 methods are suitable for clinical detection of MRCNS

and latex agglutination is the most convenient and rapid.MRCNS had a serious cross-resistance in aminoglycosides

guinolones

tetracycline

macrolides

sulfanilamide and

so the best choice for therapy is VAN.GEN

SXT

CIP

TET and CLI could be used for MSCNS

except VAN.