肿瘤基质血管内皮细胞血管形成能力研究

朱红梅; 汤为学; 张大志

doi:10.13406/j.cnki.cyxb.2005.03.017

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肿瘤基质血管内皮细胞血管形成能力研究

更新时间：2024-01-17

- 肿瘤基质血管内皮细胞血管形成能力研究
- Study on the angiogenic ability of tumor vascular endothelial cells
- 重庆医科大学学报 2005年第3期页码：369-372
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所
  2. 重庆医科大学病理生理学教研室
  3. 重庆医科大学第二临床学院感染病科,重庆,400010
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.03.017
  中图分类号： R737.31
- 纸质出版日期：2005，
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[1]朱红梅,汤为学,张大志.肿瘤基质血管内皮细胞血管形成能力研究[J].重庆医科大学学报,2005(03):369-372+479.

ZHU Hongmei, et al. Study on the angiogenic ability of tumor vascular endothelial cells[J]. Journal of Chongqing Medical University, 2005,(3):369-372.
[1]朱红梅,汤为学,张大志.肿瘤基质血管内皮细胞血管形成能力研究[J].重庆医科大学学报,2005(03):369-372+479. DOI： 10.13406/j.cnki.cyxb.2005.03.017.

ZHU Hongmei, et al. Study on the angiogenic ability of tumor vascular endothelial cells[J]. Journal of Chongqing Medical University, 2005,(3):369-372. DOI： 10.13406/j.cnki.cyxb.2005.03.017.

摘要

目的:探讨肿瘤基质血管内皮细胞的血管形成能力。方法:对卵巢癌细胞SKOV3培养上清液诱导人内皮细胞ECV304获得能稳定生长的ECV304-SKOV3细胞

用免疫细胞化学测定其血管形成因子的表达、PT-PCR测定其端粒酶活性、双室联合培养测定其游走能力与管腔形成能力

并分析其蛋白质合成能力、粘贴能力。并与亲代细胞ECV304比较。结果:ECV304-SKOV3细胞蛋白质合成能力、端粒酶活性强于亲代细胞ECV304（P<0.001）

但粘贴能力减弱（P<0.001）。表达bcl-2、基质金属蛋白酶-9（matrixmetalloproteinase9

MMP-9）增强（P<0.05）

而粘合素（tenascin

TN）减弱（P<0.05）。ECV304-SKOV3细胞较ECV304细胞的游走能力强2.10倍

差异非常显著（P<0.001）

管腔形成能力也明显增强

平均每视野管腔数分别为（12.4±1.517）条和（6.4±0.894）条（P<0.01）

管腔长分别为（262.17±49.52）μm和（137.34±18.69）μm（P<0.01）。结论:肿瘤基质血管内皮细胞存活能力增强

表达多种细胞因子与血管形成因子增强

相应地游走能力与管腔形成能力明显增强。

Abstract

Objective:To explore the angiogenic ability of vascular endothelial cells in solid tumors.Methods:Immunocytochemistry

reverse transcription polymerase chain reaction (PT-PCR)

and two-well co-culture system were used to detect the expressions of matrix metalloproteinase 9(MMP-9)

transforming growth factor-β1(TGF-β1)

tenascin(TN)and bcl-2

telomerase activity

and their ability of both migration and tube formation of ECV304-SKOV3 cells respectively

which

as the cell model

had been established in our laboratory before.Then their abilities of protein synthesis and adhesion were analysed.Results:The ability to synthesize proteins and telomerase activity was enhanced(P<0.001).Adhesion potential of ECV304-SKOV3 cells decreased (P<0.001).The expressions of MMP-9 and bcl-2 in ECV304-SKOV3 cells increased (P<0.05)

while TN decreased (P<0.05)

as compared with that of ECV304 cells.The migratory capability of ECV304-SKOV3 cells was 2.10 times greater than that of ECV304 cells (P<0.001). ECV304-SKOV3 cells formed tubular structures more and longer than did ECB304 cells

when co-cultured with SKOV3 cells (P<0.01).Conclusion:The results suggest that ECV304-SKOV3 cells

compared with parental cells ECV304

survive longer

express and secrete various cytokines and angiogeneic factors stronger

and has greater ability to migrate and form vascular structure.

关键词

肿瘤内皮细胞血管血管形成

Keywords

tumorendothelial cellVasculatureangiogenesis

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