MAPK<sup>ERK1/2</sup>信号转导途径在实验性癫痫大鼠海马结构的激活

夏杰; 陈阳美

doi:10.13406/j.cnki.cyxb.2005.03.015

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MAPK^ERK1/2信号转导途径在实验性癫痫大鼠海马结构的激活

更新时间：2024-01-17

- MAPK^ERK1/2信号转导途径在实验性癫痫大鼠海马结构的激活
- Activation of MAPK^ERK1/2 signal transduction pathway in the hippocampal formation of rat after experimental seizure
- 重庆医科大学学报 2005年第3期页码：363-365
- 作者机构：
  
  1. 重庆医科大学第二临床学院神经内科
  2. 重庆医科大学第二临床学院神经内科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.03.015
  中图分类号： R742.1
- 纸质出版日期：2005，
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[1]夏杰,陈阳美.MAPK~(ERK1/2)信号转导途径在实验性癫痫大鼠海马结构的激活[J].重庆医科大学学报,2005(03):363-365.

XIA Jie, et al. Activation of MAPK^ERK1/2 signal transduction pathway in the hippocampal formation of rat after experimental seizure[J]. Journal of Chongqing Medical University, 2005,(3):363-365.
[1]夏杰,陈阳美.MAPK~(ERK1/2)信号转导途径在实验性癫痫大鼠海马结构的激活[J].重庆医科大学学报,2005(03):363-365. DOI： 10.13406/j.cnki.cyxb.2005.03.015.

XIA Jie, et al. Activation of MAPK^ERK1/2 signal transduction pathway in the hippocampal formation of rat after experimental seizure[J]. Journal of Chongqing Medical University, 2005,(3):363-365. DOI： 10.13406/j.cnki.cyxb.2005.03.015.

摘要

目的:探讨细胞外信号调节激酶在癫痫持续状态（SE）大鼠脑内的活性变化规律及其意义。方法:建立氯化锂-匹罗卡品癫痫持续状态模型

在不同时间点用免疫组化法检测海马结构ERK1/2的磷酸化状态（p-ERK1/2）

并观察组织病理学改变。结果:生理盐水对照组p-ERK1/2在海马结构仅见于神经纤维。SE30min后ERK1/2在胞体强烈激活

2h时达到高峰

6h后各区均明显减少

12h后降至基础水平

在所有时间点

ERK1/2在齿状回的活化程度均显著高于CA1和CA3区。海马结构各区可见到许多神经元发生变性和坏死。结论:MAPKERK1/2信号转导途径在匹罗卡品致痫过程中被激活

并可能在发作后海马神经元急性损伤与保护的病理生理过程以及长时程形态学变化的形成中起作用。

Abstract

Objective:To investigate the spatiotemporal distribution pattern of active ERK1/2 in rat hippocampal formation following pilocarpine-induced status epilepticus(SE) and to discuss its significance.Methods:The lithium-pilocarpine-induced model of SE was established to evaluate the phosphorylation forms of ERK1/2(p-ERK1/2) by immunohistochemistry and observe histopathology changes at different times after pilocarpine injection.Results:The p-ERK1/2 immunoreactivity was observed only in neurites of saline-treated rats hippocampal formation.In pilocarpine-treated rats

the hippocampal neurons showed a strong immunoreactivity of p-ERK1/2 in the body at 30 min which peaked at 2h after pilocarpine injection

while decreased markedly at 6h and returned to basal level at 12h.There were more immunoreactive positive cells in dentate gyrus than those in regions of CA1 and CA3.Many injured neurons were found in hippocampus of SE rats.Conclusion:MAPK ERK1/2 signal transduction pathway can be activated in SE induced by pilocarpine

and may play a critical role in the pathophysiological process of neuron injury and protection related to seizure

and even mediate further long-lasting morphological changes.

关键词

细胞外信号调节激酶癫痫海马匹罗卡品

Keywords

ERKepilepsyHippocampal formationPilocarpine

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MAPKERK1/2信号转导途径在实验性癫痫大鼠海马结构的激活

Activation of MAPKERK1/2 signal transduction pathway in the hippocampal formation of rat after experimental seizure

DOI：10.13406/j.cnki.cyxb.2005.03.015

摘要

Abstract

关键词

Keywords

references

MAPK^ERK1/2信号转导途径在实验性癫痫大鼠海马结构的激活

Activation of MAPK^ERK1/2 signal transduction pathway in the hippocampal formation of rat after experimental seizure