脐血单个核细胞体外培养和诱导后神经干细胞标志物mRNA的表达

向静; 江德鹏; 王昌铭; 王景周

doi:10.13406/j.cnki.cyxb.2005.03.012

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脐血单个核细胞体外培养和诱导后神经干细胞标志物mRNA的表达

更新时间：2024-01-17

- 脐血单个核细胞体外培养和诱导后神经干细胞标志物mRNA的表达
- Expression of neural stem cell-specific molecules cultured in vitro and induced mononuclear cells derived from human cord blood
- 重庆医科大学学报 2005年第3期页码：352-355
- 作者机构：
  
  1. 第三军医大学大坪医院野战外科研究所神经内科
  2. 第三军医大学西南医院急诊科
  3. 第三军医大学大坪医院野战外科研究所神经内科,重庆,400042
  4. ,重庆,400038
- 作者简介：
- 基金信息：
  
  重庆市科委攻关项目（7830）
- DOI：10.13406/j.cnki.cyxb.2005.03.012
  中图分类号： R329.2
- 纸质出版日期：2005，
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[1]向静,江德鹏,王昌铭,王景周.脐血单个核细胞体外培养和诱导后神经干细胞标志物mRNA的表达[J].重庆医科大学学报,2005(03):352-355+401.

XIANG Jing, et al. Expression of neural stem cell-specific molecules cultured in vitro and induced mononuclear cells derived from human cord blood[J]. Journal of Chongqing Medical University, 2005,(3):352-355.
[1]向静,江德鹏,王昌铭,王景周.脐血单个核细胞体外培养和诱导后神经干细胞标志物mRNA的表达[J].重庆医科大学学报,2005(03):352-355+401. DOI： 10.13406/j.cnki.cyxb.2005.03.012.

XIANG Jing, et al. Expression of neural stem cell-specific molecules cultured in vitro and induced mononuclear cells derived from human cord blood[J]. Journal of Chongqing Medical University, 2005,(3):352-355. DOI： 10.13406/j.cnki.cyxb.2005.03.012.

摘要

目的:探讨人脐血单个核细胞（humancordbloodmonocytes

HCMNCs）培养增殖过程中的形态变化及诱导后神经干细胞标志物mRNA的表达。方法:采用OLYMPUS倒置显微镜观察培养过程中HCMNCs形态的变化;RT-PCR方法鉴定MNCs用神经生长因子（NGF）和维甲酸（RA）诱导前后神经干细胞（neuralstemcells

NSCs）标志物巢蛋白（Nestin）及Musashi-1蛋白mRNA的表达。结果:HCMNCs培养前

胞体较小

呈均一的圆形;培养后

细胞胞体增大

有破骨样细胞和间质样细胞两种形态。破骨样细胞胞体较大

呈圆形

多个核。间质样细胞胞体呈较均一的长梭形

形态类似于骨髓MSCs

但较骨髓MSCs稍小。RT-PCR检测提示

诱导前HCMNCs中Nestin弱表达

Musashi-1无表达;诱导后两者均表达增强

之后逐渐减弱。结论:使用密度梯度离心法可以从脐血中分离得到具有多向分化潜能的HCMNCs

经过EGF和RA诱导后

神经干细胞标志表达一过性增强后减弱。

Abstract

Objective:To investigate the configuration changes of human cord blood monocytes (HCMNCs)under specific culture conditions and the expression of NSCs marker under induction.Methods:HCMNCs were prepared from normal placenta after full term normal delivery. Observe the shape by microscope and reverse transcriptase polymerase chain reaction (RT-PCR) for nestin and musashi-1 antigen were performed to confirm nestin and musashi-1 antigen expression in HCMNCs.Results:RT-PCR demonstrated small amount of nestin expressed in HCMNCs but no musashi-1 antigen before induction

and both showed the strongest expression in 48h

then faded away gradually. The uncultivated MNCs were small and round.After culturing

the cells became larger

some cells change their shape into rhombic.Conclusion:The newly HCMNCs isolated by centrifugation over Lymphoprep densitgradient expressed nestin mRNA in low level and no musashi-1 show up

after induction

the former and musashi-1 increased intermittently and then decreased with the time of culturing.

关键词

脐血单个核细胞巢蛋白Musashi-1RT-PCR

Keywords

Human cord blood monocytesNestinMusashi-1RT-PCR

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