德氮吡格对人肝癌细胞株增殖抑制的体外研究

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德氮吡格对人肝癌细胞株增殖抑制的体外研究

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- 德氮吡格对人肝癌细胞株增殖抑制的体外研究
- Inhibition of proliferation of human hepatocellular carcinoma cell lines by TNBG in vitro
- 重庆医科大学学报 2005年第3期页码：345-347
- 作者机构：
  
  1. 重庆市生物化学与分子药理重点实验室
  2. 重庆医科大学基础医学院病理生理教研室
  3. 重庆市生物化学与分子药理重点实验室,重庆,400016
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助（批准号30371632）。
- DOI：10.13406/j.cnki.cyxb.2005.03.010
  中图分类号： R735.7
- 纸质出版日期：2005，
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[1]李龙江,陈志琼,汤为学,杨晓兰,余瑜.德氮吡格对人肝癌细胞株增殖抑制的体外研究[J].重庆医科大学学报,2005(03):345-347.

LI Longjiang, et al. Inhibition of proliferation of human hepatocellular carcinoma cell lines by TNBG in vitro[J]. Journal of Chongqing Medical University, 2005,(3):345-347.
[1]李龙江,陈志琼,汤为学,杨晓兰,余瑜.德氮吡格对人肝癌细胞株增殖抑制的体外研究[J].重庆医科大学学报,2005(03):345-347. DOI： 10.13406/j.cnki.cyxb.2005.03.010.

LI Longjiang, et al. Inhibition of proliferation of human hepatocellular carcinoma cell lines by TNBG in vitro[J]. Journal of Chongqing Medical University, 2005,(3):345-347. DOI： 10.13406/j.cnki.cyxb.2005.03.010.

摘要

目的:观察德氮吡格（TNBG）对人肝癌细胞株SMMC-7721和HepG2增殖的影响

探讨其抗肿瘤作用机制。方法:运用MTT法检测德氮吡格对肿瘤细胞增殖的影响

流式细胞仪观察细胞周期分布情况

Westernblot分析cyclinB1和p34cdc2的变化。结果:德氮吡格作用SMMC-7721和HepG2细胞72h后

能抑制细胞增殖

呈剂量依赖性。流式细胞仪检测到两株细胞均有S期和G2/M期细胞增加

并且德氮吡格还能诱导HepG2细胞发生凋亡。Westernblot发现CyclinB1表达在药物处理组明显降低

而p34cdc2则无明显改变。结论:德氮吡格能抑制肿瘤细胞增殖

可能通过调控CyclinB1表达将肿瘤细胞阻滞于G2/M期

从而达到抗肿瘤的效应。

Abstract

Objective:To investigate the effect of TNBG on proliferation of human hepatocellular carcinoma cell lines SMMC-7721 and HepG2.Methods:MTT assay was used to test the effects of anti-proliferation.The flow cytometric analysis was used to detect the cell cycle distribution.Western blot was adopted for detecting the expression of cyclinB1 and p34 cdc2 .Results:Anti-proliferation effects were observed in SMMC-7721 and HepG2 after intervention of TNBG for 72 hours

and the effects were in concentration-dependent manners.The cells showed S and G2/M arrest in both of cell lines

and apoptosis was induced only in HepG2.The expression of cyclinB1 was inhibited

while p34 cdc2 was not changed.Conclusion:TNBG can inhibit the proliferation of tumor cells

which is due to G2/M arrest caused by expression inhibition of cyclinB1 induced by TNBG.

关键词

SMMC-7721HepG2CyclinB1细胞周期增殖抑制

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