Ferumoxides标记神经干细胞及其对增殖分化的影响

王爽; 谢鹏; 牟君; 赵裕光; 贾延劼

doi:10.13406/j.cnki.cyxb.2005.03.004

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Ferumoxides标记神经干细胞及其对增殖分化的影响

更新时间：2024-01-17

- Ferumoxides标记神经干细胞及其对增殖分化的影响
- Experimental study on biological characteristics of ferumoxides labeled neural stem cells
- 重庆医科大学学报 2005年第3期页码：321-324
- 作者机构：
  
  1. 重庆医科大学临床学院神经内科
  2. 重庆医科大学临床学院普外科
  3. 重庆医科大学临床学院神经内科,重庆,400016
- 作者简介：
- 基金信息：
  
  国家自然基金项目资助（3 0 3 70 5 0 0 ）;重庆医科大学优秀博士学位论文科研经费资助;中国博士后科学基金资助项目（2003033363）
- DOI：10.13406/j.cnki.cyxb.2005.03.004
  中图分类号： R329.2
- 纸质出版日期：2005，
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[1]王爽,谢鹏,牟君,赵裕光,贾延劼.Ferumoxides标记神经干细胞及其对增殖分化的影响[J].重庆医科大学学报,2005(03):321-324.

WANG Shuang, et al. Experimental study on biological characteristics of ferumoxides labeled neural stem cells[J]. Journal of Chongqing Medical University, 2005,(3):321-324.
[1]王爽,谢鹏,牟君,赵裕光,贾延劼.Ferumoxides标记神经干细胞及其对增殖分化的影响[J].重庆医科大学学报,2005(03):321-324. DOI： 10.13406/j.cnki.cyxb.2005.03.004.

WANG Shuang, et al. Experimental study on biological characteristics of ferumoxides labeled neural stem cells[J]. Journal of Chongqing Medical University, 2005,(3):321-324. DOI： 10.13406/j.cnki.cyxb.2005.03.004.

摘要

目的:研究超顺磁性氧化铁（superparamagneticironoxides.SPIOs）标记神经干细胞及其生物学特性。方法:神经干细胞培养、传代和诱导分化;菲立磁（Ferumoxides

一种SPIOs）标记神经干细胞

制备磁标记神经干细胞;利用免疫细胞化学、透射电镜和Prussianblue染色等方法对磁标记神经干细胞的生长、分化等生物学特性进行研究。结果:在原代及传代细胞中有Nestin阳性细胞即神经干细胞。血清诱导下

神经干细胞可分化为GFAP、MAP - 2、NSE、Tau蛋白及NF2 0 0阳性细胞。Feru moxides与神经干细胞共同孵育后

透射电镜及Prussianblue染色显示胞浆中含有铁颗粒

SPIOs也可以随细胞的分裂增殖而传到子代细胞中。随Ferumoxides浓度的增高（2 .8μg/ml～16 .8μg/ml）

Ferumoxides对神经干细胞存活、分化能力的影响无显著性差异（P >0 .0 5 ）。当Ferumoxides的浓度大于2 2 .4 μg/ml时

SPIOs影响其存活和分化（P <0 .0 5 ）。结论:本实验在国内首次利用Ferumoxides作为磁标记探针

对神经干细胞进行成功磁标记。为进一步利用核磁共振（MRI）对神经干细胞活体追踪奠定实验基础。

Abstract

Objective:To study the biological characteristics of superparamgnetic iron oxides(SPIOs)labeled neural stem cells(NSCs).Methods:The culture

passage and differentiation of NSCs were completed from newborn rat brain.Ferumoxides labeled NSCs were prepared as magnetic labeled NSCs.Using immunocytochemistry

transmission electron microscopy(TEM) and Prussian blue staining

the growth

differentiation and other biological characteristics were studied.Results:Nestin positive cells were found in the culture and daughter clones.NSCs could be differentiated into positive GFAP

MAP-2

NSE

Tau protein and NF200 cells in serum culture.When NSCs incubated with ferumoxides

the iron particles were seen in intracellular(non-nucleus)as well as in dauhgter clones.With the increase in concentration of ferumoxides(2.8μg/ml～16.8μg/ml)

it showed no significant difference in effects on the growth and differentiation of NSCs(P>0.05).When the concentration of ferumoxides exceeded 22.4μg/ml

there was significant difference(P<0.05).Conclusion:In China

ferumoxides is first successfully used to label NSCs

a kind of SPIOs with magnetic labeled NSCs

which provides experimental basis for tracing magnetic labeled NSCs in vivo with MRI.

关键词

神经干细胞超顺磁性氧化铁Ferumoxides磁标记细胞

Keywords

neural stem cellsSuperparamagnetic iron oxides(SPIOs)FerumoxidesMagnetic labeled cells

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