结核菌H37Ra免疫小鼠后巨噬细胞TNF-α表达的初步探讨

陶昆; 卢贤瑜; 成海恩

doi:10.13406/j.cnki.cyxb.2005.02.010

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结核菌H37Ra免疫小鼠后巨噬细胞TNF-α表达的初步探讨

更新时间：2024-01-17

- 结核菌H37Ra免疫小鼠后巨噬细胞TNF-α表达的初步探讨
- Expression of TNF-α from celiac macrophage after immunization of H37Ra in mice
- 重庆医科大学学报 2005年第2期页码：200-202
- 作者机构：
  
  1. 重庆医科大学检验系临床免疫学教研室
  2. 重庆医科大学基础医学院分子生物学教研室,重庆,400016
- 作者简介：
- 基金信息：
  
  重庆医科大学创新基金资助项目（CX200204）
- DOI：10.13406/j.cnki.cyxb.2005.02.010
  中图分类号： R392
- 纸质出版日期：2005，
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[1]陶昆,卢贤瑜,成海恩.结核菌H37Ra免疫小鼠后巨噬细胞TNF-α表达的初步探讨[J].重庆医科大学学报,2005(02):200-202+227.

TAO Kun, et al. Expression of TNF-α from celiac macrophage after immunization of H37Ra in mice[J]. Journal of Chongqing Medical University, 2005,(2):200-202.
[1]陶昆,卢贤瑜,成海恩.结核菌H37Ra免疫小鼠后巨噬细胞TNF-α表达的初步探讨[J].重庆医科大学学报,2005(02):200-202+227. DOI： 10.13406/j.cnki.cyxb.2005.02.010.

TAO Kun, et al. Expression of TNF-α from celiac macrophage after immunization of H37Ra in mice[J]. Journal of Chongqing Medical University, 2005,(2):200-202. DOI： 10.13406/j.cnki.cyxb.2005.02.010.

摘要

目的:探讨小鼠接种结核菌H37Ra株后

巨噬细胞表达TNF -α的活性情况。方法:H37Ra皮内、腹腔途径免疫小鼠后第30、6 0天

采用生物学活性方法检测小鼠腹腔巨噬细胞表达的TNF -α活性。结果:小鼠接种H37Ra后

30天时TNF-α活性分别为2 2 .0 6U/ml（腹腔）和2 2 .6 3U/ml（皮内） ;6 0天时TNF -α的活性分别为36 .76U/ml（腹腔）和33.78U/ml（皮内）

明显高于未免疫组（P <0 .0 1） ;与BCG皮内免疫组比较

30天时无显著性差异（P >0 .0 5 ）

6 0天时有明显差异（P <0 .0 5 ）。结论:H37Ra免疫小鼠后能诱导机体产生TNF -α。

Abstract

Objective:To explore the activity of tumor necrosis factor-α(TNF-α)from celiac macrophage after immunization of H37Ra in mice.Methods:The activity of TNF-α from celiac macrophage was measured by biological method at 30 th day and 60 th day after subcutaneous immunization and celiac immunization of H37Ra in mice.Results:After immunization of H37Ra in mice

the activity of TNF-α by celiac immunized route and subcutaneous immunized route was 22.06U/ml and 22.63U/ml respectively at 30 th day;and it was 36.76U/ml and 33.78U/ml respectively at 60 th day.The activity of TNF-α immunized with H37Ra at 30 th day and 60 th day was much higher than that of non-immunization group( P <0.01).There was no statistically significant difference between H37Ra immunization group and BCG immunization group at 30 th day( P >0.05)

however

the activity of TNF-α in H37Ra immunization group was much higher than that in BCG immunization group at 60 th day( P <0.05).Conclusion:H37Ra can induce TNF-α.

关键词

H37Ra肿瘤坏死因子α结核分枝杆菌巨噬细胞

Keywords

H37RaTumor necrosis factor-αmycobacterium tuberculosismacrophage

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