LI Xing, et al. IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells[J]. Journal of Chongqing Medical University, 2005,(2):163-165. DOI: 10.13406/j.cnki.cyxb.2005.02.001.
Objective:To make the isolation and 2-DE analysis of mitochondria
in order to prepare the following proteomic study on mitochondria of hepatoma-cell. Methods:Mitochondria was separated from cell homogenate by means of density gradient centrifugation
and 2-DE was conducted to examine the protein profile of mitochondrial preparations.Results:The increase in purity of mitochondria was found to be 12 times by density gradient centrifugation.Mitochondrial proteins were displayed well in the 2-DE pattern after purification.Conclusion:The isolation procedure is practicable
which provides a basis for the following proteomic study on mitochondria.
关键词
线粒体梯度离心双向凝胶电泳
Keywords
mitochondriaDensity gradient centrifugationtwo-dimensional gel electrophoresis