Objective:To assess the feasibility of dilute sulfuric acid injected into the rabbit liver in vivo

and to provide experimental proof for the interventional therapy on hepatocellular carcinoma.Methods:10 big white rabbits were randomly divided into two groups.10% sulfuric acid was injected into liver at a dose of 1ml.In the first group

the biochemical changes in serum were examined before and immediately

3

7

and 15 days after the procedure.In the second group

histopathological findings of the liver were observed after 10% sulfuric acid injected into liver immediately

3

7

15 and 30days postoperatively.Results:The ALT and AST level in serum had transient elevation

but shortly returned to the same level as before the procedure.There was no significant elevation of ALB

ALP

γ-GGT

TBIL

DBIL and BUN level after the procedure.Pathological changes indicated hepatocellular degeneration

coagulative necrosis

inflammatory cells infiltration and granulation tissue proliferation.Conclusion:Diluted sulfuric acid have strong power of cells inactivation

but without obvious toxicity and side-effect

which could be a new type of tumor cells inactivation material for the treatment of hepatocellular carcinoma local ablation.