结核菌H37Ra免疫小鼠后PPD刺激淋巴细胞增殖转化的探讨

陶昆; 卢贤瑜; 成海恩; 徐以民

doi:10.13406/j.cnki.cyxb.2005.01.023

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结核菌H37Ra免疫小鼠后PPD刺激淋巴细胞增殖转化的探讨

更新时间：2024-01-17

- 结核菌H37Ra免疫小鼠后PPD刺激淋巴细胞增殖转化的探讨
- The proliferation of lymphocyte with stimulation of PPD after immunization with H37Ra in mice
- 重庆医科大学学报 2005年第1期页码：82-84
- 作者机构：
  
  1. 重庆医科大学检验系临床免疫教研室
  2. 重庆医科大学基础医学院分子生物学教研室
  3. 重庆医科大学计生系生殖遗传教研室,重庆,400016
- 作者简介：
- 基金信息：
  
  重庆医科大学创新基金资助项目（CX200204）
- DOI：10.13406/j.cnki.cyxb.2005.01.023
  中图分类号： R392
- 纸质出版日期：2005，
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[1]陶昆,卢贤瑜,成海恩,徐以民.结核菌H37Ra免疫小鼠后PPD刺激淋巴细胞增殖转化的探讨[J].重庆医科大学学报,2005(01):82-84.

TAO Kun, et al. The proliferation of lymphocyte with stimulation of PPD after immunization with H37Ra in mice[J]. Journal of Chongqing Medical University, 2005,(1):82-84.
[1]陶昆,卢贤瑜,成海恩,徐以民.结核菌H37Ra免疫小鼠后PPD刺激淋巴细胞增殖转化的探讨[J].重庆医科大学学报,2005(01):82-84. DOI： 10.13406/j.cnki.cyxb.2005.01.023.

TAO Kun, et al. The proliferation of lymphocyte with stimulation of PPD after immunization with H37Ra in mice[J]. Journal of Chongqing Medical University, 2005,(1):82-84. DOI： 10.13406/j.cnki.cyxb.2005.01.023.

摘要

目的探讨小鼠接种H37Ra后

PPD刺激鼠淋巴细胞的增殖转化情况。方法用H37Ra皮内、腹腔免疫小鼠后30、60天

检测小鼠脾淋巴细胞受PPD刺激后的转化率。结果小鼠接种H37Ra后

30天淋转率分别为21.98%（腹腔）和24.85%（皮内）;60天淋转率分别为25.50%（腹腔）和25.02%（皮内）

明显高于未免疫组（P<0.01）;与BCG皮内免疫组比较

无显著性差异（P>0.05）;H37Ra腹腔和皮内不同途径接种

淋巴细胞转化率无明显差异。结论H37Ra免疫小鼠后能诱导机体产生抗结核的免疫应答。

Abstract

Objective:To research the proliferation of lymphocyte with stimulation of purified protein derivative (PPD) after immunization with H37Ra in mice.Methods:The rate of lymphocyte proliferation with stimulation of PPD was determined at day 30 and day 60 after subcutaneous immunization and celiac immunization with H37Ra in mice.Results:After immunization with H37Ra in mice

the rate of lymphocyte proliferation by celiac immunization route and subcutaneous immunization route was 21.98% and 24.85% respectively at day 30;and it was 25.50% and 25.02% respectively at day 60.The rate of lymphocyte proliferation immunized with H37Ra at day 30 and day 60 were much higher than that of non-immunization( P <0.01);compared with the proliferation of lymphocyte by subcutaneous immunization route of BCG

there were no statistically significant difference( P >0.05);there were no significant difference between subcutaneous immunization route and celiac immunization route of H37Ra in mice.Conclusion:H37Ra can induce anti-tuberculosis immune response.

关键词

H37Ra淋巴细胞转化结核分枝杆菌PPD

Keywords

H37RaLymphocyte proliferationmycobacterium tuberculosisPPD

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