小鼠胚泡黏附时已黏附侧与未黏附侧子宫内膜蛋白质组差异的研究

潘卫; 王应雄; 杨戎

doi:10.13406/j.cnki.cyxb.2004.05.004

您当前的位置：

首页 >

文章列表页 >

小鼠胚泡黏附时已黏附侧与未黏附侧子宫内膜蛋白质组差异的研究

更新时间：2024-01-17

- 小鼠胚泡黏附时已黏附侧与未黏附侧子宫内膜蛋白质组差异的研究
- Comparative study between protein profile of mice endometrium with blastocysts adhering and without blastocysts adhering
- 重庆医科大学学报 2004年第5期页码：572-575
- 作者机构：
  
  1. 重庆医科大学生殖医学系遗传优生教研室
  2. 重庆医科大学生殖医学系遗传优生教研室,重庆,400016
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（3 0 2 70 5 10 ）
- DOI：10.13406/j.cnki.cyxb.2004.05.004
  中图分类号： R321.3
- 纸质出版日期：2004，
扫描看全文
[1]潘卫,王应雄,杨戎.小鼠胚泡黏附时已黏附侧与未黏附侧子宫内膜蛋白质组差异的研究[J].重庆医科大学学报,2004(05):572-575.

PAN Wei, et al. Comparative study between protein profile of mice endometrium with blastocysts adhering and without blastocysts adhering[J]. Journal of Chongqing Medical University, 2004,(5):572-575.
[1]潘卫,王应雄,杨戎.小鼠胚泡黏附时已黏附侧与未黏附侧子宫内膜蛋白质组差异的研究[J].重庆医科大学学报,2004(05):572-575. DOI： 10.13406/j.cnki.cyxb.2004.05.004.

PAN Wei, et al. Comparative study between protein profile of mice endometrium with blastocysts adhering and without blastocysts adhering[J]. Journal of Chongqing Medical University, 2004,(5):572-575. DOI： 10.13406/j.cnki.cyxb.2004.05.004.

摘要

目的 :用蛋白质组相关技术分析小鼠胚泡黏附时已粘附侧与未黏附侧子宫内膜蛋白质组的差异

探讨小鼠胚泡在双侧子宫黏附不同步现象发生的可能机制。方法 :用固相 pH梯度双向凝胶电泳（2D -PAGE）分别分离妊娠五天（D5）小鼠胚泡已黏附侧与未黏附侧子宫内膜的蛋白质组。银染显色

PDQuest 2DE软件分析。结果 :图像分析测得两种胶的匹配率达 82 .5 %

在等电点pI4～ 7、分子量 14 .0～ 75 .4kD范围内分离得胚泡黏附侧小鼠子宫内膜蛋白点大约 76 0个

胚泡未黏附侧小鼠子宫内膜蛋白点大约 72 0个

其中至少 6 0个蛋白点在两种子宫内膜间有 2倍以上的量变。结论 :小鼠双侧子宫内膜蛋白质的差异表达

是造成两侧子宫内膜胚泡附着不同步的一个可能原因

Abstract

Objective:Examining the difference in the protein profiling of endometrium between the blastocyst-adhering side and the side without blastocyst adhering

in order to study the asynchrony of blastocysts adhesion between two uteri.Methods:The endometrium with and without blastocyst adhering from the mice pregnant for 5 days were analyzed by two-dimensional gel electrophoresis and imaging analysis.Results:The number of matched protein spots between two gels was up to 82.5%.760 and 720 protein spots with a molecular mass between 14.0～75.4 and isoelectric points (pI)from 4～7 were catalogued from silver-stained gels of the blastocyst-adhering endometrium and the endometrium without blastocyst adhering respectively.At least 60 proteins were up-regulated or down-regulated by over two folds between two groups.Conclusion:the different expression of protein profile may be a cause underlying the the asynchrony of blastocysts adhesion between two uteri.

关键词

蛋白质组双向凝胶电泳胚泡着床

Keywords

Proteometwo-dimensional gel electrophoresisBlastocystImplantation

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

HeLa细胞蛋白质组双向电泳技术的建立

双向电泳分析精索静脉曲张不育患者精浆蛋白质的表达差异

辅助生殖技术发展对围产医学及子代健康的挑战

小鼠囊胚在人羊膜上着床行为的实验研究

超声波对小鼠胚胎表达整合素及其着床能力的影响