Objective:To investigate the relationship among inflammatory reaction and cytokine levels

nitric oxide（NO） content in aqueous humor after alkali burn.Methods:12 adult New Zealand rabbits were divided into two groups（6 rabbits each）:normal control group and alkali burn experimental group.Aqueous humor samples were aspirated on the alkali burn 1

3

7

14 and 30 days

and the total number of white blood cells in the aqueous humor and their subset distribution as well as tumor necrosis factor-α （TNF-α） and NO

-

2

/NO

-

3

were counted.The data were analyzed using analysis of variance of SPSS software.Results:There was a higher number of inflammatory cells and a higher level of TNF-α and NO

-

2

/NO

-

3

in alkali burn eyes than that in the normal control eyes.The number of mononuclear phagocyte and the levels of TNF-α

the content of NO

-

2

/NO

-

3

in aqueous humor were the highest in alkali burn group in 7～14 days.The change of TNF-α

NO

-

2

/NO

-

3

and inflammatory reaction were basically similar

that is

the more serious the reaction

the higher the levels of the content.Conclusion:The intraocular inflammation after alkali burn is closely related to the change in cytokine levels and NO content in aqueous humor.