Genistein对人卵巢癌细胞系SKOV<sub>3</sub>诱导内皮细胞迁移的作用及机制探讨

李昱; 米粲

doi:10.13406/j.cnki.cyxb.2004.04.009

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Genistein对人卵巢癌细胞系SKOV₃诱导内皮细胞迁移的作用及机制探讨

更新时间：2024-01-17

- Genistein对人卵巢癌细胞系SKOV₃诱导内皮细胞迁移的作用及机制探讨
- A study on the effect of Genistein on migration of endothelial cells induced by human ovarian carcinoma cell line SKOV₃
- 重庆医科大学学报 2004年第4期页码：437-440
- 作者机构：
  
  1. 重庆医科大学基础医学院病理教研室
  2. 重庆医科大学基础医学院病理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.009
  中图分类号： R737.31
- 纸质出版日期：2004，
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[1]李昱,米粲.Genistein对人卵巢癌细胞系SKOV_3诱导内皮细胞迁移的作用及机制探讨[J].重庆医科大学学报,2004(04):437-440.

LI Yu, et al. A study on the effect of Genistein on migration of endothelial cells induced by human ovarian carcinoma cell line SKOV₃[J]. Journal of Chongqing Medical University, 2004,(4):437-440.
[1]李昱,米粲.Genistein对人卵巢癌细胞系SKOV_3诱导内皮细胞迁移的作用及机制探讨[J].重庆医科大学学报,2004(04):437-440. DOI： 10.13406/j.cnki.cyxb.2004.04.009.

LI Yu, et al. A study on the effect of Genistein on migration of endothelial cells induced by human ovarian carcinoma cell line SKOV₃[J]. Journal of Chongqing Medical University, 2004,(4):437-440. DOI： 10.13406/j.cnki.cyxb.2004.04.009.

摘要

目的 :研究Genistein对人卵巢浆液性乳头状囊腺癌细胞系SKOV3 诱导人内皮细胞系ECV - 30 4迁移的作用

以探讨Genistein抑制血管生成作用的机理。方法 :采用微孔滤膜培养小室及双室联合细胞培养方法

观察在 0 .5 %BS培养条件下

SKOV3 细胞或其条件培养基诱导的ECV - 30 4细胞迁移以及Genistein对SKOV3 细胞或其条件培养基诱导的ECV - 30 4细胞迁移的影响 ;用免疫组化方法检测血管生长因子VEGF、bFGF及TGFβ - 1蛋白的表达水平。结果 :SKOV3 细胞或其条件培养基对内皮细胞均有较强的趋化作用

Genistein对SKOV3 细胞或其条件培养基诱导的ECV - 30 4细胞迁移有呈剂量依赖性的抑制作用。 2 0 μmol/LGenistein处理ECV - 30 4细胞 4 8h后

VEGF、bFGF蛋白的表达水平降低

而TGFβ - 1蛋白的表达水平升高。结论 :Genistein可明显抑制人卵巢浆液性乳头状囊腺癌细胞系SKOV3 及其条件培养基对人内皮细胞系ECV- 30 4细胞的诱导迁移作用 ;Genistein可能通过抑制促血管生成调节因子VEGF和bFGF的蛋白表达

增强血管生成的负性调节因子TGFβ - 1的蛋白表达来抑制这种迁移诱导作用

Abstract

Objective:To investigate the effect of Genistein on migration of human endothelial cells ECV-304 induced by human ovarian serous cystadenocarcinoma cell line SKOV

and to explore the mechanism of anti-angiogenesis of Genistein.Methods:Millicell chamber and coculture method were used to observe the influence on migration of ECV-304 induced by SKOV

cells or its conditioned media

and the effect of Genistein on migration of ECV-304 induced by SKOV

cells or its conditioned media without serum.The expression of angiogenesis associated protein VEGF

bFGF and TGFβ-1 were determined using immunocytochemical method.Results:Either SKOV

cells or its conditioned media could induce the committed chemotactic migration of ECV-304.The chemotactic migrations of ECV-304 induced by SKOV

or its conditioned media were significantly inhibited by Genistein in a dose-dependent manner.20μmol/L Genistein could down-regulate the expression of VEGF and bFGF;up-regulate the expression of TGFβ-1.Conclusion:Migrations of ECV-304 induced by human ovarian carcinoma cell line SKOV

or its conditioned media were apparently inhibited by Genistein.It suggests that this inhibitory effect of Genistein is completed by down-regulating the expression of vessel growth-promoting factor VEGF and bFGF

and up-regulating the expression of negative regulator TGFβ-1.This may be one of the mechanisms of anti-angiogenesis of Genistein.

关键词

Genistein卵巢肿瘤迁移内皮细胞

Keywords

Genisteinovarian tumormigrationendothelial cell

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Genistein对人卵巢癌细胞系SKOV3诱导内皮细胞迁移的作用及机制探讨

A study on the effect of Genistein on migration of endothelial cells induced by human ovarian carcinoma cell line SKOV3

DOI：10.13406/j.cnki.cyxb.2004.04.009

摘要

Abstract

关键词

Keywords

references

Genistein对人卵巢癌细胞系SKOV₃诱导内皮细胞迁移的作用及机制探讨

A study on the effect of Genistein on migration of endothelial cells induced by human ovarian carcinoma cell line SKOV₃